A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles
文献类型：期刊论文
| 作者 | Xue, Wangxin; Zhang, Guanxin; Zhang, Deqing |
| 刊名 | ANALYST
 |
| 出版日期 | 2011 |
| 卷号 | 136期号:15页码:3136-3141 |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c1an15224f |
| 英文摘要 | Gold nanoparticles (Au-NPs) with negative charges aggregate in the presence of Arg(6) due to electrostatic interactions resulting in the red-shift of the plasmon absorption. But, after incubation of Arg(6) with trypsin the aggregation of Au-NPs can be prohibited. Accordingly, a newly designed-Au-NPs based colorimetric assay method for trypsin activity is established. Trypsin with a concentration as low as 1.6 ng mL(-1) can be assayed with this new colorimetric assay. This colorimetric label-free assay for trypsin can be performed in aqueous solution and both Au-NPs and Arg(6) are easily accessible. Thus, this assay method is useful for screening inhibitors of trypsin. |
| 语种 | 英语 |
| WOS记录号 | WOS:000292972200014 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71931]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Deqing |
| 作者单位 | Chinese Acad Sci, Organ Solids Lab, Beijing Natl Lab Mol Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Wangxin,Zhang, Guanxin,Zhang, Deqing. A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles[J]. ANALYST,2011,136(15):3136-3141. |
| APA | Xue, Wangxin,Zhang, Guanxin,&Zhang, Deqing.(2011).A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles.ANALYST,136(15),3136-3141. |
| MLA | Xue, Wangxin,et al."A sensitive colorimetric label-free assay for trypsin and inhibitor screening with gold nanoparticles".ANALYST 136.15(2011):3136-3141. |
入库方式： OAI收割
来源：化学研究所
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