Simple and Sensitive Method for Detecting Point Mutations of Epidermal Growth Factor Receptor Using Cationic Conjugated Polymers
文献类型：期刊论文
| 作者 | Yang, Qiong1; Qiu, Tian2,3; Wu, Wei1; Zhu, Chunlei1; Liu, Libing1; Ying, Jianming2,3; Wang, Shu1 |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2011-11-01 |
| 卷号 | 3期号:11页码:4539-4545 |
| 关键词 | Conjugated Polymers Egfr Dna Mutation Fret Fluorescent Sensor Mutation Detection |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/am201248y |
| 英文摘要 | The L858R mutation of epidermal growth factor receptor (EGFR) in nonsmall cell lung cancer is associated with the increased sensitivity to EGFR tyrosine kinase inhibitors. In this paper, a simple and sensitive method for identification of L858R mutation in cell lines and tumor tissues was developed using cationic conjugated polymer-based fluorescence resonance energy transfer technology (CCP-based FRET). The new detection system can detect even as low as 4-8% mutation of the total DNA. Through the detection results for 48 DNA samples from tumor tissues, a sensitivity of 95.24% (20/21) and a specificity of 96.30% (26/27) were demonstrated. Further, the application of this method in clinical molecular diagnosis was validated by detecting T790 M in EGFR of 35 patients. In comparison with DNA sequencing and real-time PCR methods, our new protocol simplifies procedures by eliminating the need for primer labeling, cumbersome workups and sophisticated instruments and improves sensitivity by amplifying fluorescence signals. Our CCP-based FRET technology is particularly attractive because of its higher sensitivity, cost-effective, and simple characteristics. Particularly, this new method could confirm the suspected positive samples arisen by DNA sequencing and real-time PCR methods. Thus, the CCP-based FRET technology opens up an avenue for clinical therapy by guiding medication to lung cancer patients responsive to anti-EGFR therapy. |
| 语种 | 英语 |
| WOS记录号 | WOS:000297195500053 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/71985]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Qiong |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China 2.Chinese Acad Med Sci, Canc Hosp, Dept Pathol, Beijing 100021, Peoples R China 3.Peking Union Med Coll, Beijing 100021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Qiong,Qiu, Tian,Wu, Wei,et al. Simple and Sensitive Method for Detecting Point Mutations of Epidermal Growth Factor Receptor Using Cationic Conjugated Polymers[J]. ACS APPLIED MATERIALS & INTERFACES,2011,3(11):4539-4545. |
| APA | Yang, Qiong.,Qiu, Tian.,Wu, Wei.,Zhu, Chunlei.,Liu, Libing.,...&Wang, Shu.(2011).Simple and Sensitive Method for Detecting Point Mutations of Epidermal Growth Factor Receptor Using Cationic Conjugated Polymers.ACS APPLIED MATERIALS & INTERFACES,3(11),4539-4545. |
| MLA | Yang, Qiong,et al."Simple and Sensitive Method for Detecting Point Mutations of Epidermal Growth Factor Receptor Using Cationic Conjugated Polymers".ACS APPLIED MATERIALS & INTERFACES 3.11(2011):4539-4545. |
入库方式： OAI收割
来源：化学研究所
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