A novel dendritic surfactant for enhanced microcystin-LR detection by double amplification in a quartz crystal microbalance biosensor
文献类型：期刊论文
| 作者 | Xia, Yuetong; Zhang, Jianping; Jiang, Long |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2011-08-01 |
| 卷号 | 86期号:1页码:81-86 |
| 关键词 | Microcystin-lr Quartz Crystal Microbalance Dendritic Surfactant Vesicle Array Double Amplification |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2011.03.019 |
| 英文摘要 | Enhanced sensitivity for the hepatotoxin microcystin-LR (MC-LR) was achieved in a quartz crystal microbalance (QCM) system via double amplification. For primary amplification, an innovative interface on the QCM was obtained as a matrix by the vesicle layer formed by our synthetic dendritic surfactant, bin (amidoethyl-carbamoylethyl) octadecylamine (C18N3). The vesicle matrix was then functionalised by an optimised concentration of monoclonal antibodies against MC-LR (anti-MC-LR) to detect the analyte. The results showed that a detection limit of 100 ng/mL was achieved by primary amplification. To achieve higher sensitivity, secondary amplification was implemented with anti-MC-LR gold nanoparticle (AuNPs) conjugates as probes, which lowered the detection limit for MC-LR to 1 ng/mL (the maximum concentration recommended by the World Health Organization [WHO] in drinking water for humans). The QCM immunosensor reported here has advantages such as high sensitivity, portability, simplicity, and cost-effectiveness for MC-LR detection. It would be uniquely superior compared with current MC-LR detection techniques for on-the-spot water detection. Furthermore, the methodology described here is also potentially significant in many fields for the routine monitoring of environmental and food safety. (C) 2011 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000291458100011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72013]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jiang, Long |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia, Yuetong,Zhang, Jianping,Jiang, Long. A novel dendritic surfactant for enhanced microcystin-LR detection by double amplification in a quartz crystal microbalance biosensor[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2011,86(1):81-86. |
| APA | Xia, Yuetong,Zhang, Jianping,&Jiang, Long.(2011).A novel dendritic surfactant for enhanced microcystin-LR detection by double amplification in a quartz crystal microbalance biosensor.COLLOIDS AND SURFACES B-BIOINTERFACES,86(1),81-86. |
| MLA | Xia, Yuetong,et al."A novel dendritic surfactant for enhanced microcystin-LR detection by double amplification in a quartz crystal microbalance biosensor".COLLOIDS AND SURFACES B-BIOINTERFACES 86.1(2011):81-86. |
入库方式： OAI收割
来源：化学研究所
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