Method for detection of Hg2+ based on the specific thymine-Hg2+-thymine interaction in the DNA hybridization on the surface of quartz crystal microbalance
文献类型：期刊论文
| 作者 | Sheng, Zhonghan2; Han, Jianhua2; Zhang, Jianping2; Zhao, Hong1; Jiang, Long2 |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2011-10-15 |
| 卷号 | 87期号:2页码:289-292 |
| 关键词 | Quartz Crystal Microbalance Hg2++++ Ion Gold Nanoparticle Thymine |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2011.05.031 |
| 英文摘要 | A simple and sensitive method was developed for the detection of mercury ions with quartz crystal microbalance (QCM), based on the specific thymine-Hg2(+)-thymine (T-Hg2+-T) interaction and gold nanoparticle-mediated signal amplification. To enhance the sensitivity of detection a sandwich hybridization approach was adopted in this work. The QCM gold surface was modified with the probe SH-oligonucleotides (Oligo-1) and 6-Mercapto-1-hexanol to form an active surface for the hybridization of a longer ss-DNA (Oligo-2), and then Oligo-3 hybridazated with an excess and matching part of Oligo-2. In all oligonucleotides, there existed T bases. In the presence of Hg2+ ions, special T-Hg2+-T reaction greatly enhanced the hybridization of oligonucleotides and detection sensitivity. The gold nanoparticle (Au NPs) amplifier method further increased the sensitivity of detection. A detection sensitivity of 5 nM Hg2+ was obtained in the QCM system, whereas other coexisting metal ions (such as Ni2+, Mg2+, Co2+, Cr3+, Pb2+, Cd2+, Mn2+, Ba2+) had no significant interference. This method reveals a new approach for the manufacture of a kind of simple and low cost sensors for the Hg2+ detection. (C) 2011 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000294142900011 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72289]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Hong |
| 作者单位 | 1.Chinese Acad Sci, Grad Univ, Coll Chem & Chem Engn, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Chem, Key Lab Colloid & interface Sci, BNLMS, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sheng, Zhonghan,Han, Jianhua,Zhang, Jianping,et al. Method for detection of Hg2+ based on the specific thymine-Hg2+-thymine interaction in the DNA hybridization on the surface of quartz crystal microbalance[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2011,87(2):289-292. |
| APA | Sheng, Zhonghan,Han, Jianhua,Zhang, Jianping,Zhao, Hong,&Jiang, Long.(2011).Method for detection of Hg2+ based on the specific thymine-Hg2+-thymine interaction in the DNA hybridization on the surface of quartz crystal microbalance.COLLOIDS AND SURFACES B-BIOINTERFACES,87(2),289-292. |
| MLA | Sheng, Zhonghan,et al."Method for detection of Hg2+ based on the specific thymine-Hg2+-thymine interaction in the DNA hybridization on the surface of quartz crystal microbalance".COLLOIDS AND SURFACES B-BIOINTERFACES 87.2(2011):289-292. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。