Direct screening of G-quadruplex ligands from Kalopanax septemlobus (Thunb.) Koidz extract by high performance liquid chromatography
文献类型：期刊论文
| 作者 | Bai, Ge1; Cao, Xueli1; Zhang, Hong2,3; Xiang, Junfeng2; Ren, Hong1; Tan, Li2; Tang, Yalin2 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2011-09-16 |
| 卷号 | 1218期号:37页码:6433-6438 |
| 关键词 | G-quadruplex Ligands High-performance Liquid Chromatography (Hplc) High-speed Countercurrent Chromatography (Hsccc) Kalopanax Septemlobus (Thunb.) Koidz Antitumor Activity |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2011.07.028 |
| 英文摘要 | G-quadruplex DNA structure is considered to be a very attractive target for antitumor drug design due to its unique role in maintaining telomerase activities. Therefore, discovering ligands with high stability of G-quadruplex structure is of great interest. In this paper, high-performance liquid chromatography (HPLC) was used for fast screening of G-quadruplex ligands from the crude extract of Kalopanax septemlobus (Thunb.) Koidz, a traditional Chinese medicine. Four potent G-quadruplex ligands were firstly selected through HPLC by comparing the peak profiles and absorption intensity of the crude sample before and after interaction with G-quadruplex DNA. Then the target compounds were isolated and purified by high-speed countercurrent chromatography (HSCCC) for further confirmation of their stabilities of G-quadruplex by temperature-dependent circular dichroism (CD). Four compounds were isolated and identified as 2,4-dihydroxybenzoic acid (I), chlorogenic acid (II), caffeic acid (III) and 5-feruloylquinic acid (IV) each by MS and NMR. Finally, compound I, II, Ill were each proved to be potent G-quadruplex ligands by decreasing the peak intensity in HPLC chromatogram after complexation with G-quadruplex, which stabilize G-quadruplex by 7 +/- 2 degrees C, 10 +/- 2 degrees C, and 3 +/- 2 degrees C respectively, based on CD analyses. However, compound IV showed no G-quadruplex stability. The decrease of peak absorption intensity in HPLC chromatogram is the most important signal to find G-quadruplex ligands. This provides a very promising strategy for fast screening G-quadruplex ligands from natural plant extracts. (C) 2011 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000295113600026 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72337]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Cao, Xueli |
| 作者单位 | 1.Beijing Technol & Business Univ, Beijing Key Lab Plant Resources Res & Dev, Beijing 100048, Peoples R China 2.Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing Natl Lab Mol Sci,Ctr Mol Sci, Beijing 100190, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Ge,Cao, Xueli,Zhang, Hong,et al. Direct screening of G-quadruplex ligands from Kalopanax septemlobus (Thunb.) Koidz extract by high performance liquid chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2011,1218(37):6433-6438. |
| APA | Bai, Ge.,Cao, Xueli.,Zhang, Hong.,Xiang, Junfeng.,Ren, Hong.,...&Tang, Yalin.(2011).Direct screening of G-quadruplex ligands from Kalopanax septemlobus (Thunb.) Koidz extract by high performance liquid chromatography.JOURNAL OF CHROMATOGRAPHY A,1218(37),6433-6438. |
| MLA | Bai, Ge,et al."Direct screening of G-quadruplex ligands from Kalopanax septemlobus (Thunb.) Koidz extract by high performance liquid chromatography".JOURNAL OF CHROMATOGRAPHY A 1218.37(2011):6433-6438. |
入库方式： OAI收割
来源：化学研究所
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