Single-Molecule Analysis of PIP2;1 Dynamics and Partitioning Reveals Multiple Modes of Arabidopsis Plasma Membrane Aquaporin Regulation
文献类型：期刊论文
| 作者 | Li, Xiaojuan1,2; Wang, Xiaohua1,2; Yang, Yong3; Li, Ruili1,2; He, Qihua4; Fang, Xiaohong3; Doan-Trung Luu5; Maurel, Christophe5; Lin, Jinxing1 |
| 刊名 | PLANT CELL
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 23期号:10页码:3780-3797 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.111.091454 |
| 英文摘要 | PIP2;1 is an integral membrane protein that facilitates water transport across plasma membranes. To address the dynamics of Arabidopsis thaliana PIP2;1 at the single-molecule level as well as their role in PIP2;1 regulation, we tracked green fluorescent protein-PIP2;1 molecules by variable-angle evanescent wave microscopy and fluorescence correlation spectroscopy (FCS). Single-particle tracking analysis revealed that PIP2;1 presented four diffusion modes with large dispersion of diffusion coefficients, suggesting that partitioning and dynamics of PIP2;1 are heterogeneous and, more importantly, that PIP2;1 can move into or out of membrane microdomains. In response to salt stress, the diffusion coefficients and percentage of restricted diffusion increased, implying that PIP2;1 internalization was enhanced. This was further supported by the decrease in PIP2;1 density on plasma membranes by FCS. We additionally demonstrated that PIP2;1 internalization involves a combination of two pathways: a tyrphostin A23-sensitive clathrin-dependent pathway and a methyl-beta-cyclodextrin-sensitive, membrane raft-associated pathway. The latter was efficiently stimulated under NaCl conditions. Taken together, our findings demonstrate that PIP2;1 molecules are heterogeneously distributed on the plasma membrane and that clathrin and membrane raft pathways cooperate to mediate the subcellular trafficking of PIP2;1, suggesting that the dynamic partitioning and recycling pathways might be involved in the multiple modes of regulating water permeability. |
| 语种 | 英语 |
| WOS记录号 | WOS:000298175900018 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72341]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Lin, Jinxing |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Mol Physiol, Inst Bot, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100190, Peoples R China 4.Peking Univ Hlth Sci Ctr, Beijing 100191, Peoples R China 5.Univ Montpellier 2, Inst Biol Integrat Plantes, Unite Mixte Rech Ctr Natl Rech Sci 5004, Unite Mixte Rech Inst Natl Rech Agron 0386, F-34060 Montpellier 2, France |
| 推荐引用方式 GB/T 7714 | Li, Xiaojuan,Wang, Xiaohua,Yang, Yong,et al. Single-Molecule Analysis of PIP2;1 Dynamics and Partitioning Reveals Multiple Modes of Arabidopsis Plasma Membrane Aquaporin Regulation[J]. PLANT CELL,2011,23(10):3780-3797. |
| APA | Li, Xiaojuan.,Wang, Xiaohua.,Yang, Yong.,Li, Ruili.,He, Qihua.,...&Lin, Jinxing.(2011).Single-Molecule Analysis of PIP2;1 Dynamics and Partitioning Reveals Multiple Modes of Arabidopsis Plasma Membrane Aquaporin Regulation.PLANT CELL,23(10),3780-3797. |
| MLA | Li, Xiaojuan,et al."Single-Molecule Analysis of PIP2;1 Dynamics and Partitioning Reveals Multiple Modes of Arabidopsis Plasma Membrane Aquaporin Regulation".PLANT CELL 23.10(2011):3780-3797. |
入库方式： OAI收割
来源：化学研究所
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