A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance
文献类型：期刊论文
| 作者 | Zhang, Meng1,2; Lin, Shixian1; Song, Xinwen2; Liu, Jun1; Fu, Ye3; Ge, Xi2; Fu, Xinmiao2,4; Chang, Zengyi2,4; Chen, Peng R.1,4,5 |
| 刊名 | NATURE CHEMICAL BIOLOGY
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 7期号:10页码:671-677 |
| ISSN号 | 1552-4450 |
| DOI | 10.1038/NCHEMBIO.644 |
| 英文摘要 | Acid chaperones are essential factors in preserving the protein homeostasis for enteric pathogens to survive in the extremely acidic mammalian stomach (pH 1-3). The client proteins of these chaperones remain largely unknown, primarily because of the exceeding difficulty of determining protein-protein interactions under low-pH conditions. We developed a genetically encoded, highly efficient protein photocrosslinking probe, which enabled us to profile the in vivo substrates of a major acid-protection chaperone, HdeA, in Escherichia coli periplasm. Among the identified HdeA client proteins, the periplasmic chaperones DegP and SurA were initially found to be protected by HdeA at a low pH, but they subsequently facilitated the HdeA-mediated acid recovery of other client proteins. This unique, ATP-independent chaperone cooperation in the ATP-deprived E. coli periplasm may support the acid resistance of enteric bacteria. The crosslinker would be valuable in unveiling the physiological interaction partners of any given protein and thus their functions under normal and stress conditions. |
| 语种 | 英语 |
| WOS记录号 | WOS:000295027100010 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72395]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Peng R. |
| 作者单位 | 1.Peking Univ, Beijing Natl Lab Mol Sci, Coll Chem & Mol Engn, Beijing 100871, Peoples R China 2.Peking Univ, State Key Lab Prot & Plant Gene Res, Sch Life Sci, Beijing 100871, Peoples R China 3.Univ Chicago, Dept Chem, Chicago, IL 60637 USA 4.Peking Univ, Ctr Prot Sci, Beijing 100871, Peoples R China 5.Peking Tsinghua Ctr Life Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Meng,Lin, Shixian,Song, Xinwen,et al. A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance[J]. NATURE CHEMICAL BIOLOGY,2011,7(10):671-677. |
| APA | Zhang, Meng.,Lin, Shixian.,Song, Xinwen.,Liu, Jun.,Fu, Ye.,...&Chen, Peng R..(2011).A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance.NATURE CHEMICAL BIOLOGY,7(10),671-677. |
| MLA | Zhang, Meng,et al."A genetically incorporated crosslinker reveals chaperone cooperation in acid resistance".NATURE CHEMICAL BIOLOGY 7.10(2011):671-677. |
入库方式： OAI收割
来源：化学研究所
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