Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation
文献类型：期刊论文
| 作者 | Cui, Yi-Ran1; Hong, Chao2; Zhou, Ying-Lin1; Li, Yue3; Gao, Xiao-Ming2,3; Zhang, Xin-Xiang1 |
| 刊名 | TALANTA
 |
| 出版日期 | 2011-09-15 |
| 卷号 | 85期号:3页码:1246-1252 |
| 关键词 | Fe3o4/au Magnetic Nanoparticles (Fe3o4@au Mnps) Oriented Immobilization Antibody Protein a Cell Separation |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2011.05.010 |
| 英文摘要 | Orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles were synthesized for cell separation. The Fe3O4@Au magnetic nanoparticles were synthesized by reducing HAuCl4 on the surfaces of Fe3O4 nanoparticles, which were further characterized in detail by TEM, XRD and UV-vis spectra. Anti-CD3 monoclonal antibody was orientedly bioconjugated to the surface of Fe3O4@Au nanoparticles through affinity binding between the Fc portion of the antibody and protein A that covalently immobilized on the nanoparticles. The oriented immobilization method was performed to compare its efficiency for cell separation with the non-oriented one, in which the antibody was directly immobilized onto the carboxylated nanoparticle surface. Results showed that the orientedly bioconjugated Fe3O4@Au MNPs successfully pulled down CD3(+) T cells from the whole splenocytes with high efficiency of up to 98.4%, showing a more effective cell-capture nanostructure than that obtained by non-oriented strategy. This developed strategy for the synthesis and oriented bioconjugation of Fe3O4@Au MNPs provides an efficient tool for cell separation, and may be further applied to various fields of bioanalytical chemistry for diagnosis, affinity extraction and biosensor. (C) 2011 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000294092800005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72433]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhang, Xin-Xiang |
| 作者单位 | 1.Peking Univ, BNLMS, Key Lab Biochem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem, Beijing 100871, Peoples R China 2.Soochow Univ, Inst Biol & Med Sci, Suzhou 215123, Peoples R China 3.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100191, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Yi-Ran,Hong, Chao,Zhou, Ying-Lin,et al. Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation[J]. TALANTA,2011,85(3):1246-1252. |
| APA | Cui, Yi-Ran,Hong, Chao,Zhou, Ying-Lin,Li, Yue,Gao, Xiao-Ming,&Zhang, Xin-Xiang.(2011).Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation.TALANTA,85(3),1246-1252. |
| MLA | Cui, Yi-Ran,et al."Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation".TALANTA 85.3(2011):1246-1252. |
入库方式： OAI收割
来源：化学研究所
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