Rapid and sensitive detection of DNA polymerase fidelity by singly labeled smart fluorescent probes
文献类型：期刊论文
| 作者 | Song, Chen; Zhang, Chen; Zhao, Meiping |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2011-01-15 |
| 卷号 | 26期号:5页码:2699-2702 |
| 关键词 | Dna Polymerase Fidelity Singly Labeled Smart Fluorescent Probe Real-time Monitoring Mispair Extension Proofreading Activity |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/j.bios.2010.08.073 |
| 英文摘要 | We report here a novel approach to monitor the DNA polymerase fidelity in detailed steps, including mispair extension, mispair formation and 3' -> 5' proofreading. The method is based on the photo-induced electron transfer between the natural base guanine and the labeled fluorophore. The G:T mispair extension catalyzed by the exonuclease-deficient Klenow fragment DNA polymerase (KF exo(-)) was easily detected and the effect of the nearest neighbor base pair on the mispair extension rate was clearly observed. More importantly, kinetics of the G:T, G:A and G:G mispair formation and extension under single turnover conditions were measured by continuous fluorescence-based assay for the first time. The probes also showed their applicability to discriminate the 3' -> 5' proofreading activity of different exonuclease-proficient DNA polymerases. The presented method may greatly simplify the screening and characterization procedures of the increasing number of polymerases that are thought to be potential targets for drug design and cancer treatment. It will also provide important information for deep understanding of the polymerase fidelity mechanism. (C) 2010 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000286904400149 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72461]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Meiping |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, Key Lab Bioorgan Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Song, Chen,Zhang, Chen,Zhao, Meiping. Rapid and sensitive detection of DNA polymerase fidelity by singly labeled smart fluorescent probes[J]. BIOSENSORS & BIOELECTRONICS,2011,26(5):2699-2702. |
| APA | Song, Chen,Zhang, Chen,&Zhao, Meiping.(2011).Rapid and sensitive detection of DNA polymerase fidelity by singly labeled smart fluorescent probes.BIOSENSORS & BIOELECTRONICS,26(5),2699-2702. |
| MLA | Song, Chen,et al."Rapid and sensitive detection of DNA polymerase fidelity by singly labeled smart fluorescent probes".BIOSENSORS & BIOELECTRONICS 26.5(2011):2699-2702. |
入库方式： OAI收割
来源：化学研究所
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