In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection
文献类型：期刊论文
| 作者 | Bai, Yu; Du, Fuyou; Yang, Youyou; Bai, Yu; Liu, Huwei |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 34期号:20页码:2893-2900 |
| 关键词 | Ce Non-covalent Labeling Plant Peptide Hormone Quantum Dots Systemin |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201100551 |
| 英文摘要 | Quantum dots (QDs), with their superior size-dependent fluorescence properties, have been employed as non-covalent fluorescent labels for the determination of tomato systemin (TomSys) by capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection. The optimum conditions of in-capillary labeling and CE separation were investigated in detail, and complete separation of QDs-labeled TomSys from free QDs labels was achieved. Satisfactory results were obtained in terms of linearity (R(2) = 0.998), sensitivity (limit of detection, 66 fmol) and repeatability (run-to-run RSDs of migration time and peak area, 0.9 and 4.6%, respectively; day-to-day RSDs of migration time and peak area, 3.1 and 11.9%, respectively). The established CE-LIF method was later applied in the detection of TomSys spiked in the sample of tomato leaves, which showed the applicability of the proposed method in the analysis of the target plant peptide hormone in the complex matrix. |
| 语种 | 英语 |
| WOS记录号 | WOS:000296905400015 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72535]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Huwei |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci,Minist Educ, Key Lab Bioorgan Chem & Mol Engn,Inst Analyt Chem, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bai, Yu,Du, Fuyou,Yang, Youyou,et al. In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection[J]. JOURNAL OF SEPARATION SCIENCE,2011,34(20):2893-2900. |
| APA | Bai, Yu,Du, Fuyou,Yang, Youyou,Bai, Yu,&Liu, Huwei.(2011).In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection.JOURNAL OF SEPARATION SCIENCE,34(20),2893-2900. |
| MLA | Bai, Yu,et al."In-capillary non-covalent labeling and determination of tomato systemin with quantum dots in capillary electrophoresis with laser-induced fluorescence detection".JOURNAL OF SEPARATION SCIENCE 34.20(2011):2893-2900. |
入库方式： OAI收割
来源：化学研究所
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