The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme
文献类型：期刊论文
| 作者 | Wang, Xinyi1,2; Xu, Yao1; Chen, Yang1; Li, Limei2; Liu, Feng1; Li, Na1 |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 400期号:7页码:2085-2091 |
| 关键词 | Gold Nanoparticles Aggregation Lysozyme Dna Aptamer Plasmon Resonance Light Scattering Visual Detection |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-011-4943-1 |
| 英文摘要 | We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering (PRLS) measurement and naked-eye detection using for the first time the lysozyme DNA aptamer as the recognition element. Lysozyme DNA aptamer could stabilize gold nanoparticles (AuNPs) at high ionic strength. Introducing lysozyme to the system easily triggered the aggregation of AuNPs, producing a red-to-blue color change of the solution, red-shifted plasmon absorption, and enhanced plasmon resonance light scattering. The linear range was found to be 0.2 similar to 4 nM for 0.7 nM AuNPs, 0.3 similar to 6 nM for 1.4 nM AuNPs and 0.6 similar to 8 nM for 2.1 nM AuNPs. About 0.1 nM lysozyme can produce an observable enhancement of PRLS signal. For visual detection, 1 nM lysozyme can produce a very distinctive color change. Satisfactory recoveries were obtained for simulated saliva and diluted urine samples, indicating that the method has potential for analyses of clinical samples. The simplicity and high sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine analysis. |
| 语种 | 英语 |
| WOS记录号 | WOS:000290801000028 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72577]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, Na |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Minist Educ, Key Lab Bioorgan Chem & Mol Engn,BNLMS, Beijing 100871, Peoples R China 2.Shenyang Agr Univ, Coll Sci, Shenyang 110161, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xinyi,Xu, Yao,Chen, Yang,et al. The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,400(7):2085-2091. |
| APA | Wang, Xinyi,Xu, Yao,Chen, Yang,Li, Limei,Liu, Feng,&Li, Na.(2011).The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,400(7),2085-2091. |
| MLA | Wang, Xinyi,et al."The gold-nanoparticle-based surface plasmon resonance light scattering and visual DNA aptasensor for lysozyme".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 400.7(2011):2085-2091. |
入库方式： OAI收割
来源：化学研究所
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