Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE
文献类型：期刊论文
| 作者 | Xia Zhi-Ning1,2; Li Li-Xian1,2; Chen Hua2; Xiong Cai-Qiao3; Yang Feng-Qing2 |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2011-04-10 |
| 卷号 | 32期号:4页码:851-856 |
| 关键词 | Plug-plug Kinetic Capillary Electrophoresis (Ppkce) Cell Membrane Kinetic Parameter Interaction |
| ISSN号 | 0251-0790 |
| 英文摘要 | Based on the theory of plug-plug kinetic capillary electrophoresis(ppKCE), the rabbit red blood cell membrane and human red blood cell membrane were used to study the kinetic parameters of the interaction between the cell membrane and drug. The rate constants of complex formation k(on), the dissociation k(off) and the binding constant K(b) between cell membrane and drugs were directly determined simultaneously. The stability of peak height and migration time was studied. The influence of pH and temperature on the kinetic parameters of the interaction between the cell membrane and drugs were examined and analyzed. The results indicate that at physiological pH value, the cell membrane showed biggest biology activeness, the k(on) and K(b) between cell membrane and drugs reached maximum value. The values of k(off), k(on) and K(b) between cell membrane and CIT, Epinastine, Gatifloxacin and Roxatidine were gained. The accuracy of ppKCE was confirmed by the time ratio method and UV-Vis absorption spectra. It could provide a new approach for the evaluation of drug efficiency, toxicity and pharmacokinetics in vivo. |
| 语种 | 英语 |
| WOS记录号 | WOS:000290076100011 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72709]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Xia Zhi-Ning |
| 作者单位 | 1.Chongqing Univ, Coll Bioengn, Chongqing 400030, Peoples R China 2.Chongqing Univ, Coll Chem & Chem Engn, Chongqing 400030, Peoples R China 3.Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xia Zhi-Ning,Li Li-Xian,Chen Hua,et al. Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2011,32(4):851-856. |
| APA | Xia Zhi-Ning,Li Li-Xian,Chen Hua,Xiong Cai-Qiao,&Yang Feng-Qing.(2011).Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,32(4),851-856. |
| MLA | Xia Zhi-Ning,et al."Kinetic Parameters of Interaction Between Cell Membrane and Drug by ppKCE".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 32.4(2011):851-856. |
入库方式： OAI收割
来源：化学研究所
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