Ratiometric Fluorescent Sensor Based on Inhibition of Resonance for Detection of Cadmium in Aqueous Solution and Living Cells
文献类型：期刊论文
| 作者 | Xue, Lin1; Li, Guoping1,3; Liu, Qing1,3; Wang, Huanhuan1,3; Liu, Chun2; Ding, Xunlei1; He, Shenggui1; Jiang, Hua1 |
| 刊名 | INORGANIC CHEMISTRY
 |
| 出版日期 | 2011-04-18 |
| 卷号 | 50期号:8页码:3680-3690 |
| ISSN号 | 0020-1669 |
| DOI | 10.1021/ic200032e |
| 英文摘要 | Although cadmium has been recognized as a highly toxic heavy metal and poses many detrimental effects on human health, the Cd2+-uptake and nosogenesis mechanisms are still insufficiently understood, mainly because of the lack of facile analytical methods for monitoring changes in the environmental and intracellular Cd2+ concentrations with high spatial and temporal reliability. To this end, we present the design, synthesis, and photophysical properties of a cadmium sensor, DQCd1 based on the fluorophore 4-isobutoxy-6-(dimethylamino)-8-methoxyquinaldine (model compound 1). Preliminary investigations indicate that 1 could be protonated under neutral media and yield a resonance process over the quinoline fluorophore. Upon excitation at 405 nm, 1 shows a strong fluorescence emission at 554 nm with a quantum yield of 0.17. Similarly, DQCd1 bears properties comparable to its precursor. It exhibits fluorescence emission at 558 nm (Phi(f) = 0.15) originating from the monocationic species under physiological conditions. Coordination with Cd2+ causes quenching of the emission at 558 nm and simultaneously yields a significant hypsochromic shift of the emission maximum to 495 nm (Phi(f) = 0.11) due to inhibition of the resonance process. Thus, a single-excitation, dual-emission ratiometric measurement with a large blue shift in emission (Delta lambda = 63 nm) and remarkable changes in the ratio (F-495 nm/F-558 nm) of the emission intensity (R/R-0 up to 15-fold) is established. Moreover, the sensor DQCd1 exhibits very high sensitivity for Cd2+ (K-d = 41 pM) and excellent selectivity response for Cd2+ over other heavy- and transition-metal ions and Na+, K+, Mg2+ and Ca2+ at the millimolar level. Therefore, DQCd1 can act as a ratiometric fluorescent sensor for Cd2+ through inhibition of the resonance process. Confocal microscopy and cytotoxicity experiments indicate that DQCd1 is cell-permeable and noncytotoxic under our experimental conditions. It can indeed visualize the changes of intracellular Cd2+ in living cells using dual-emission ratiometry. |
| 语种 | 英语 |
| WOS记录号 | WOS:000290457700060 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72717]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jiang, Hua |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Dalian Univ Technol, Dept Chem Engn, State Key Lab Fine Chem, Dalian 116012, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Lin,Li, Guoping,Liu, Qing,et al. Ratiometric Fluorescent Sensor Based on Inhibition of Resonance for Detection of Cadmium in Aqueous Solution and Living Cells[J]. INORGANIC CHEMISTRY,2011,50(8):3680-3690. |
| APA | Xue, Lin.,Li, Guoping.,Liu, Qing.,Wang, Huanhuan.,Liu, Chun.,...&Jiang, Hua.(2011).Ratiometric Fluorescent Sensor Based on Inhibition of Resonance for Detection of Cadmium in Aqueous Solution and Living Cells.INORGANIC CHEMISTRY,50(8),3680-3690. |
| MLA | Xue, Lin,et al."Ratiometric Fluorescent Sensor Based on Inhibition of Resonance for Detection of Cadmium in Aqueous Solution and Living Cells".INORGANIC CHEMISTRY 50.8(2011):3680-3690. |
入库方式： OAI收割
来源：化学研究所
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