EPR Effect of Amphiphilic Copolymer Micelles Observed by Fluorescent Imaging
文献类型：期刊论文
| 作者 | Liu Tong-jun1; Liu Shi2; Hu Xiu-li2; Sheng Shi-hou1; Huang Yu-bin2; Jing Xia-bin2 |
| 刊名 | CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
 |
| 出版日期 | 2011-07-01 |
| 卷号 | 27期号:4页码:628-634 |
| 关键词 | Enhanced Permeability And Retention(Epr) Effect Fluorescent Imaging Micelle Passive Targeting |
| ISSN号 | 1005-9040 |
| 英文摘要 | Enhanced permeation and retention(EPR) targeting effect of rhodamine B labeled PEG-b-P(LA-co-DHP) [PEG: poly(ethylene glycol); LA: L-lactide; DHP: 2,2-dihydroxylmethyl-propylene carbonate] micelles(RhB-micelles) was observed in H22 liver cancer bearing mice. The RhB-micelles were prepared by conjugating rhodamine B with the DHP units of amphiphilic block copolymer PEG-b-P(LA-co-DHP) followed by subsequent self-assembling of the conjugate. The parent copolymer PEG-b-P(LA-co-DHP) was synthesized by ring-opening copolymerization of LA and DHP with PEG as macroinitiator and diethyl zinc(ZnEt(2)) as catalyst. The micelles have a spherical shape and the average diameter is ca. 50 nm by TEM(transmission electron microscope) or 80 nm by DLS(dynamic light scattering). Their in vitro cell uptake experiment by CLSM(confocal laser scanning microscopy) and flow cytometry showed preferential internalization of micelles by MCF-7 human breast cancer cells to free RhB. The in vivo tests by live animal imaging and ex vivo excised organ imaging showed that after vena tail injection, free RhB molecules were distributed in the whole body through the circulation system and then gradually metabolized and excreted and there was no preferential partition in tumor bed from the beginning to the end. But the RhB-micelles were preferentially distributed to the tumor bed so that their concentration(fluorescent intensity) in tumor bed got the level of the liver at a certain time point between 1 and 6 h and reached a maximum relative intensity at around 12 h, indicating an obvious EPR effect of RhB-micelles in H22 liver cancer. |
| 语种 | 英语 |
| WOS记录号 | WOS:000294379100023 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/72725]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu Tong-jun |
| 作者单位 | 1.Jilin Univ, Dept Colorectal & Anal Surg, China Japan Union Hosp, Changchun 130033, Peoples R China 2.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu Tong-jun,Liu Shi,Hu Xiu-li,et al. EPR Effect of Amphiphilic Copolymer Micelles Observed by Fluorescent Imaging[J]. CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2011,27(4):628-634. |
| APA | Liu Tong-jun,Liu Shi,Hu Xiu-li,Sheng Shi-hou,Huang Yu-bin,&Jing Xia-bin.(2011).EPR Effect of Amphiphilic Copolymer Micelles Observed by Fluorescent Imaging.CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,27(4),628-634. |
| MLA | Liu Tong-jun,et al."EPR Effect of Amphiphilic Copolymer Micelles Observed by Fluorescent Imaging".CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 27.4(2011):628-634. |
入库方式： OAI收割
来源：化学研究所
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