Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy
文献类型:期刊论文
作者 | Xue, Lin1; Fang, Zhangjian1; Li, Guoping1,2; Wang, Huanhuan1,2; Jiang, Hua1 |
刊名 | SENSORS AND ACTUATORS B-CHEMICAL
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出版日期 | 2011-08-10 |
卷号 | 156期号:1页码:410-415 |
关键词 | Zinc Fluorescent Sensor Two Photon Intracellular Imaging |
ISSN号 | 0925-4005 |
DOI | 10.1016/j.snb.2011.04.066 |
英文摘要 | We report two fluorescent Zn(2+) sensors, AD1 and AD2. In aqueous solution, upon addition of Zn(2+). AD1 only showed the enhancement on the fluorescence intensity, while AD2, acting as a ratiometric sensor, demonstrated the variations not only on the fluorescence intensity but also on the emission wavelengths. Both sensors exhibit high sensitivity (nM level) and selectivity for Zn(2+) over Na(+), K(+), Mg(2+), and Ca(2+) at the millimolar level. Moreover, AD2 undergoes significant increase of the TPA cross sections (delta(max)) at 840 nm by the Zn(2+)-coordination induced ICT enhancement. The laser scanning confocal microscopy experiments revealed that AD2 is cell-permeable and can indeed visualize the changes of intracellular Zn(2+) in living cells through a ratiometric approach by utilizing two-photon excitation. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved. |
语种 | 英语 |
WOS记录号 | WOS:000292058200059 |
出版者 | ELSEVIER SCIENCE SA |
源URL | [http://ir.iccas.ac.cn/handle/121111/72907] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Jiang, Hua |
作者单位 | 1.Chinese Acad Sci, Inst Chem, CAS Key Lab Photochem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Xue, Lin,Fang, Zhangjian,Li, Guoping,et al. Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy[J]. SENSORS AND ACTUATORS B-CHEMICAL,2011,156(1):410-415. |
APA | Xue, Lin,Fang, Zhangjian,Li, Guoping,Wang, Huanhuan,&Jiang, Hua.(2011).Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy.SENSORS AND ACTUATORS B-CHEMICAL,156(1),410-415. |
MLA | Xue, Lin,et al."Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy".SENSORS AND ACTUATORS B-CHEMICAL 156.1(2011):410-415. |
入库方式: OAI收割
来源:化学研究所
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