中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy

文献类型:期刊论文

作者Xue, Lin1; Fang, Zhangjian1; Li, Guoping1,2; Wang, Huanhuan1,2; Jiang, Hua1
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2011-08-10
卷号156期号:1页码:410-415
关键词Zinc Fluorescent Sensor Two Photon Intracellular Imaging
ISSN号0925-4005
DOI10.1016/j.snb.2011.04.066
英文摘要We report two fluorescent Zn(2+) sensors, AD1 and AD2. In aqueous solution, upon addition of Zn(2+). AD1 only showed the enhancement on the fluorescence intensity, while AD2, acting as a ratiometric sensor, demonstrated the variations not only on the fluorescence intensity but also on the emission wavelengths. Both sensors exhibit high sensitivity (nM level) and selectivity for Zn(2+) over Na(+), K(+), Mg(2+), and Ca(2+) at the millimolar level. Moreover, AD2 undergoes significant increase of the TPA cross sections (delta(max)) at 840 nm by the Zn(2+)-coordination induced ICT enhancement. The laser scanning confocal microscopy experiments revealed that AD2 is cell-permeable and can indeed visualize the changes of intracellular Zn(2+) in living cells through a ratiometric approach by utilizing two-photon excitation. Crown Copyright (C) 2011 Published by Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000292058200059
出版者ELSEVIER SCIENCE SA
源URL[http://ir.iccas.ac.cn/handle/121111/72907]  
专题中国科学院化学研究所
通讯作者Jiang, Hua
作者单位1.Chinese Acad Sci, Inst Chem, CAS Key Lab Photochem, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Xue, Lin,Fang, Zhangjian,Li, Guoping,et al. Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy[J]. SENSORS AND ACTUATORS B-CHEMICAL,2011,156(1):410-415.
APA Xue, Lin,Fang, Zhangjian,Li, Guoping,Wang, Huanhuan,&Jiang, Hua.(2011).Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy.SENSORS AND ACTUATORS B-CHEMICAL,156(1),410-415.
MLA Xue, Lin,et al."Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy".SENSORS AND ACTUATORS B-CHEMICAL 156.1(2011):410-415.

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来源:化学研究所

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