Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery
文献类型：期刊论文
| 作者 | Lee, Yuhan1,2; Lee, Soo Hyeon1,2; Kim, Jee Seon1,2; Maruyama, Atsushi3,4; Chen, Xuesi5; Park, Tae Gwan1,2 |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2011-10-10 |
| 卷号 | 155期号:1页码:3-10 |
| 关键词 | Gold Nanoparticles Polyethylenimine Catechol Sirna Gene Delivery |
| ISSN号 | 0168-3659 |
| DOI | 10.1016/j.jconrel.2010.09.009 |
| 英文摘要 | Development of nano-sized gene delivery vehicles for small interfering RNA (siRNA) delivery is of great importance for their clinical applications such as cancer therapy. Herein, we demonstrate the controlled synthesis of polyethyleneimine (PEI)-coated gold nanoparticles (AuNPs) using catechol-conjugated PEI (PEI-C) for siRNA delivery. Since the conjugated catechol groups are reductive and moderately hydrophobic. PEI-C formed spherical multi-cored micelles in aqueous solution and served as reductive templates for the growth and synthesis of spherical AuNPs with tunable sizes and surface charges. PEI-C was stably anchored on the surface of growing crystal gold seeds with crosslinking, resulting in robust cationic AuNPs. The fabricated PEI-coated AuNPs formed stable complexes with siRNA, and the complexes showed an excellent gene silencing effect in cancer cells. Size and surface charge values of the synthesized AuNPs had a great influence on intracellular uptake and unpacking of siRNA, and the resultant gene silencing efficiency. The PEI-coated AuNPs exhibited an extremely low cytotoxicity due to the reduced density of primary amine groups and the absence of uncomplexed PEI fraction in aqueous solution. (C) 2010 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000295998200003 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/73031]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Park, Tae Gwan |
| 作者单位 | 1.Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea 2.Korea Adv Inst Sci & Technol, Grad Sch Nanosci & Technol, Taejon 305701, South Korea 3.Kyushu Univ, Inst Mat Chem & Engn, Higashi Ku, Fukuoka 8128581, Japan 4.JST, CREST, Higashi Ku, Fukuoka 8128581, Japan 5.Chinese Acad Sci, Changchun Inst Appl Chem, Grad Sch, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lee, Yuhan,Lee, Soo Hyeon,Kim, Jee Seon,et al. Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery[J]. JOURNAL OF CONTROLLED RELEASE,2011,155(1):3-10. |
| APA | Lee, Yuhan,Lee, Soo Hyeon,Kim, Jee Seon,Maruyama, Atsushi,Chen, Xuesi,&Park, Tae Gwan.(2011).Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery.JOURNAL OF CONTROLLED RELEASE,155(1),3-10. |
| MLA | Lee, Yuhan,et al."Controlled synthesis of PEI-coated gold nanoparticles using reductive catechol chemistry for siRNA delivery".JOURNAL OF CONTROLLED RELEASE 155.1(2011):3-10. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。