中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
An immunoassay in which magnetic beads act both as collectors and sensitive amplifiers for detecting antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) system

文献类型:期刊论文

作者Han, Jianhua; Zhang, Jianping; Xia, Yuetong; Li, Shaohua; Jiang, Long
刊名COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
出版日期2011-04-20
卷号379期号:1-3页码:2-9
关键词Magnetic Beads Microfluidic Chips Immunoassay Qcm
ISSN号0927-7757
DOI10.1016/j.colsurfa.2010.11.066
英文摘要An immunoassay based on the enrichment of antibodies by magnetic beads (MB) and their subsequent detection of antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) was developed and investigated. The MB conjugated to goat anti-human-immunoglobulin G (MB-anti-h-IgG) were concentrated within microchannels by the application of a magnetic field, which also functioned as a separation switch outside the MFC. The conjugation of the MB and the anti-h-IgG antibody in the MFC was completed in 1 min. Removal of the magnetic field allowed the resulting tributary buffer solution to flush the MB-anti-h-IgG out of the MFC and onto a QCM chip. The combination of the MB-anti-h-IgG and the QCM chip amplified the detection sensitivity of antigen(s) to 10(-1) ng/ml. By controlling the enrichment velocity and sample injection mode in the MFC, a linear response ranging from 10(-1) to 10(3) ng/ml was obtained. This technique establishes a simple, rapid MFC method for the amplification of a QCM-based immunoassay. (C) 2010 Elsevier B.V. All rights reserved.
语种英语
WOS记录号WOS:000289920200002
出版者ELSEVIER SCIENCE BV
源URL[http://ir.iccas.ac.cn/handle/121111/73229]  
专题中国科学院化学研究所
通讯作者Jiang, Long
作者单位Chinese Acad Sci, Inst Chem, Key Lab Colloid & Interface Sci, BNLMS, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714
Han, Jianhua,Zhang, Jianping,Xia, Yuetong,et al. An immunoassay in which magnetic beads act both as collectors and sensitive amplifiers for detecting antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) system[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2011,379(1-3):2-9.
APA Han, Jianhua,Zhang, Jianping,Xia, Yuetong,Li, Shaohua,&Jiang, Long.(2011).An immunoassay in which magnetic beads act both as collectors and sensitive amplifiers for detecting antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) system.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,379(1-3),2-9.
MLA Han, Jianhua,et al."An immunoassay in which magnetic beads act both as collectors and sensitive amplifiers for detecting antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) system".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 379.1-3(2011):2-9.

入库方式: OAI收割

来源:化学研究所

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