An immunoassay in which magnetic beads act both as collectors and sensitive amplifiers for detecting antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) system
文献类型:期刊论文
作者 | Han, Jianhua; Zhang, Jianping; Xia, Yuetong; Li, Shaohua; Jiang, Long |
刊名 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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出版日期 | 2011-04-20 |
卷号 | 379期号:1-3页码:2-9 |
关键词 | Magnetic Beads Microfluidic Chips Immunoassay Qcm |
ISSN号 | 0927-7757 |
DOI | 10.1016/j.colsurfa.2010.11.066 |
英文摘要 | An immunoassay based on the enrichment of antibodies by magnetic beads (MB) and their subsequent detection of antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) was developed and investigated. The MB conjugated to goat anti-human-immunoglobulin G (MB-anti-h-IgG) were concentrated within microchannels by the application of a magnetic field, which also functioned as a separation switch outside the MFC. The conjugation of the MB and the anti-h-IgG antibody in the MFC was completed in 1 min. Removal of the magnetic field allowed the resulting tributary buffer solution to flush the MB-anti-h-IgG out of the MFC and onto a QCM chip. The combination of the MB-anti-h-IgG and the QCM chip amplified the detection sensitivity of antigen(s) to 10(-1) ng/ml. By controlling the enrichment velocity and sample injection mode in the MFC, a linear response ranging from 10(-1) to 10(3) ng/ml was obtained. This technique establishes a simple, rapid MFC method for the amplification of a QCM-based immunoassay. (C) 2010 Elsevier B.V. All rights reserved. |
语种 | 英语 |
WOS记录号 | WOS:000289920200002 |
出版者 | ELSEVIER SCIENCE BV |
源URL | [http://ir.iccas.ac.cn/handle/121111/73229] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Jiang, Long |
作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Colloid & Interface Sci, BNLMS, Beijing 100190, Peoples R China |
推荐引用方式 GB/T 7714 | Han, Jianhua,Zhang, Jianping,Xia, Yuetong,et al. An immunoassay in which magnetic beads act both as collectors and sensitive amplifiers for detecting antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) system[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2011,379(1-3):2-9. |
APA | Han, Jianhua,Zhang, Jianping,Xia, Yuetong,Li, Shaohua,&Jiang, Long.(2011).An immunoassay in which magnetic beads act both as collectors and sensitive amplifiers for detecting antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) system.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,379(1-3),2-9. |
MLA | Han, Jianhua,et al."An immunoassay in which magnetic beads act both as collectors and sensitive amplifiers for detecting antigens in a microfluidic chip (MFC)-quartz crystal microbalance (QCM) system".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 379.1-3(2011):2-9. |
入库方式: OAI收割
来源:化学研究所
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