Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection
文献类型：期刊论文
| 作者 | Wang, Bing; Yang, Qiong; Liu, Libing; Wang, Shu |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2011-06-15 |
| 卷号 | 85期号:1页码:8-11 |
| 关键词 | Dna Conjugated Polymers Fret Fluorescent Sensors |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2010.10.026 |
| 英文摘要 | The development of methods for DNA detection is of importance in disease diagnosis, gene-targeted drug discovery and molecular biology field. In this paper, we synthesize a new cationic water-soluble CP containing fluorene moiety and flexible ethylenic moiety in the backbone (PRO for label-free DNA detection. The conformational freedom of PFV provides stronger interactions with double-stranded DNA (dsDNA) and optimizes the orientation of transition moments between PFV and ethidium bromide (EB) intercalated in dsDNA. The efficient FRET from PFV (donor) to EB (acceptor) intercalated in dsDNA is observed and the emission of EB is amplified by the good light-harvesting ability of conjugated polymers. The interactions between PFV and DNA can also be probed by measuring the FRET ratio between PFV and EB intercalated in DNA. In comparison to other DNA detection assays based on FRET and conjugated polymers, synthesis of dye-labeled DNA probe is avoided in our method, which significantly reduces the cost and the synthetic complexity. The PFV/dsDNA/EB system provides promising applications on DNA detection with a simply, fast and label-free manner. (C) 2010 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000290196100003 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/73337]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, Qiong |
| 作者单位 | Chinese Acad Sci, Inst Chem, Key Lab Organ Solids, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Bing,Yang, Qiong,Liu, Libing,et al. Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2011,85(1):8-11. |
| APA | Wang, Bing,Yang, Qiong,Liu, Libing,&Wang, Shu.(2011).Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection.COLLOIDS AND SURFACES B-BIOINTERFACES,85(1),8-11. |
| MLA | Wang, Bing,et al."Direct energy transfer from conjugated polymer to DNA intercalated dye: Label-free fluorescent DNA detection".COLLOIDS AND SURFACES B-BIOINTERFACES 85.1(2011):8-11. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。