Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme
文献类型：期刊论文
| 作者 | Zhang, Guoqing; Cao, Qing; Li, Na; Li, Kean; Liu, Feng |
| 刊名 | TALANTA
 |
| 出版日期 | 2011-02-15 |
| 卷号 | 83期号:5页码:1515-1520 |
| 关键词 | Tris(Hydroxymethyl)Aminomethane Magnetic Microspheres Protein Purification Lysozyme Chicken Egg White |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/j.talanta.2010.11.040 |
| 英文摘要 | A novel affinity purification method for lysozyme (LZM) based on functionalized magnetic microspheres was developed. Tris(hydroxymethyl)aminomethane (Tris)-modified magnetic microspheres with specific affinity toward LZM were prepared using Tris as ligand and silica-coated magnetic microshperes as support. Transmission electron microscopy and magnetic property measurement results showed that the Tris-modified magnetic microspheres have a very good core-shell structure and high magnetization. The maximum binding capacity of LZM was about 108.6 mg/g magnetic microspheres. LZM purified from chicken egg white had high purity and well-maintained activity of 8140 U/mg. This magnetic-mediated LZM purification strategy has advantages of high efficiency, low cost and easy operation. (C) 2010 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000287110100032 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/73453]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, Feng |
| 作者单位 | Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn,Minist Educ, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Guoqing,Cao, Qing,Li, Na,et al. Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme[J]. TALANTA,2011,83(5):1515-1520. |
| APA | Zhang, Guoqing,Cao, Qing,Li, Na,Li, Kean,&Liu, Feng.(2011).Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme.TALANTA,83(5),1515-1520. |
| MLA | Zhang, Guoqing,et al."Tris(hydroxymethyl)aminomethane-modified magnetic microspheres for rapid affinity purification of lysozyme".TALANTA 83.5(2011):1515-1520. |
入库方式： OAI收割
来源：化学研究所
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