Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5
文献类型：期刊论文
| 作者 | Li, Xun; Yin, Yandong; Yang, Xinxing; Zhi, Zeyong; Zhao, Xin Sheng1 |
| 刊名 | CHEMICAL PHYSICS LETTERS
 |
| 出版日期 | 2011-09-15 |
| 卷号 | 513期号:4-6页码:271-275 |
| ISSN号 | 0009-2614 |
| DOI | 10.1016/j.cplett.2011.08.017 |
| 英文摘要 | Fluorescence resonance energy transfer (FRET) has been widely used as molecular rulers to measure the distance in biomolecules. In this Letter, double stranded DNA (dsDNA) with Cy3 and Cy5 labeled on the 5'-terminals of each strand is used to investigate how temperature influences the interaction between dsDNA and Cy3/Cy5. By applying the orientation-dependent model to fit our FRET data, we found that the interaction between dsDNA and Cy3/Cy5 is so strong that Cy3 and Cy5 are mostly capped on the end of dsDNA duplex in temperature range of 4-50 degrees C. So, the orientation factor has to be cautiously considered when using FRET to measure distance. Meanwhile, with the increase of temperature, the orientational randomization of the dyes becomes more prominent. (C) 2011 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000294491500021 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/73713]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, Xin Sheng |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Dept Biol Chem, Beijing Natl Lab Mol Sci,State Key Lab Struct Che, Beijing 100871, Peoples R China 2.Peking Univ, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xun,Yin, Yandong,Yang, Xinxing,et al. Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5[J]. CHEMICAL PHYSICS LETTERS,2011,513(4-6):271-275. |
| APA | Li, Xun,Yin, Yandong,Yang, Xinxing,Zhi, Zeyong,&Zhao, Xin Sheng.(2011).Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5.CHEMICAL PHYSICS LETTERS,513(4-6),271-275. |
| MLA | Li, Xun,et al."Temperature dependence of interaction between double stranded DNA and Cy3 or Cy5".CHEMICAL PHYSICS LETTERS 513.4-6(2011):271-275. |
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来源：化学研究所
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