Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens
文献类型：期刊论文
| 作者 | Lin, Shixian1; Zhang, Zhenrun1; Xu, Hao3; Li, Lin3; Chen, She3; Li, Jie1; Hao, Ziyang1; Chen, Peng R.1,2 |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2011-12-21 |
| 卷号 | 133期号:50页码:20581-20587 |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/ja209008w |
| 英文摘要 | Enteric bacterial pathogens are known to effectively pass through the extremely acidic mammalian stomachs and cause infections in the small and/or large intestine of human hosts. However, their acid-survival strategy and pathogenesis mechanisms remain elusive, largely due to the lack of tools to directly monitor and manipulate essential components (e.g., defense proteins or invasive toxins) participating in these processes. Herein, we have extended the pyrrolysine-based genetic code expansion strategy for encoding unnatural amino acids in enteric bacterial species, including enteropathogenic Escherichia coli, Shigella, and Salmonella. Using this system, a photo-cross-linking amino acid was incorporated into a Shigella acid chaperone HdeA (shHdeA), which allowed the identification of a comprehensive list of in vivo client proteins that are protected by shHdeA upon acid stress. To further demonstrate the application of our strategy, an azide-bearing amino acid was introduced into a Shigella type 3 secretion effector, OspF, without interruption of its secretion efficiency. This site-specifically installed azide handle allowed the facile detection of OspF's secretion in bacterial extracellular space. Taken together, these bioorthogonal functionalities we incorporated into enteric pathogens were shown to facilitate the investigation of unique and important proteins involved in the pathogenesis and stress-defense mechanisms of pathogenic bacteria that remain exceedingly difficult to study using conventional methodologies. |
| 语种 | 英语 |
| WOS记录号 | WOS:000298713600081 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/73769]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Peng R. |
| 作者单位 | 1.Peking Univ, Coll Chem & Mol Engn, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China 2.Ctr Life Sci, Beijing 100871, Peoples R China 3.Natl Inst Biol Sci NIBS, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Shixian,Zhang, Zhenrun,Xu, Hao,et al. Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2011,133(50):20581-20587. |
| APA | Lin, Shixian.,Zhang, Zhenrun.,Xu, Hao.,Li, Lin.,Chen, She.,...&Chen, Peng R..(2011).Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,133(50),20581-20587. |
| MLA | Lin, Shixian,et al."Site-Specific Incorporation of Photo-Cross-Linker and Bioorthogonal Amino Acids into Enteric Bacterial Pathogens".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 133.50(2011):20581-20587. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。