Formation and characteristics of an unusual lambda-DNA species
文献类型：期刊论文
| 作者 | Cao, EH; Sun, XG; Bai, CL; Liu, CQ; He, YJ; Wang, XW; Fang, Y |
| 刊名 | BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL
 |
| 出版日期 | 1997-10-01 |
| 卷号 | 43期号:3页码:541-550 |
| 关键词 | Lambda-dna Triple-stranded Dna Melting Temperature Cd Spectra Fluorescence Spectrum Transmission Electron Microscope Atomic Force Microscope |
| ISSN号 | 1039-9712 |
| 英文摘要 | An unusual DNA species, termed as DNA species A, has been isolated and purified from thermal-denatured lambda-DNA Hind III by Sephadex G-200 gel filtration. Our studies indicate that DNA species A is resistant to DNase I digestion and has a higher melting point. The new DNA species showed a lower absorbency at 260 nm, and a lower fluorescence quantum yield after interaction with ethidium bromide (EB) than native double-stranded lambda-DNA. CD spectrum of DNA species A consists of a broad positive band centered at 245 nm and a weak negative band at 220 nm. transmission electron microscope (TEM) visualizations showed that their lengths of DNA species A fell mainly in three regions (300-500 nm, 750-1000 nm and 1500 nm) that corresponded to three fluorescence bands in the EB-stained gels. Their apparent width and height were 65-75 nm and 2.2 nm respectively as observed by images of atomic force microscope (AFM). |
| 语种 | 英语 |
| WOS记录号 | WOS:000073191000009 |
| 出版者 | ACADEMIC PRESS AUST |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/74159]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Cao, EH |
| 作者单位 | 1.Acad Sinica, Inst Biophys, Beijing 100101, Peoples R China 2.Acad Sinica, Inst Chem, Beijing 100080, Peoples R China 3.Acad Sinica, Inst Zool, Kunming 650223, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, EH,Sun, XG,Bai, CL,et al. Formation and characteristics of an unusual lambda-DNA species[J]. BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL,1997,43(3):541-550. |
| APA | Cao, EH.,Sun, XG.,Bai, CL.,Liu, CQ.,He, YJ.,...&Fang, Y.(1997).Formation and characteristics of an unusual lambda-DNA species.BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL,43(3),541-550. |
| MLA | Cao, EH,et al."Formation and characteristics of an unusual lambda-DNA species".BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL 43.3(1997):541-550. |
入库方式： OAI收割
来源：化学研究所
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