Selection of affinity ligands for protein purification from proteolytic digests
文献类型：期刊论文
| 作者 | Yang, YH; Chase, HA; Liu, GQ |
| 刊名 | BIOTECHNOLOGY TECHNIQUES
 |
| 出版日期 | 1998-03-01 |
| 卷号 | 12期号:3页码:245-251 |
| ISSN号 | 0951-208X |
| 英文摘要 | A simple method for the selection of affinity ligands from proteolytic digests by affinity chromatography is proposed. A small proportion of the peptides in the trypsin digest of bovine serum albumin (BSA) or the pepsin digest of cytochrome are retarded on insulin-immobilised or HSA (human serum albumin)-immobilised affinity columns, respectively. The peptides in these selected fractions can be immobilised onto solid phases and used in affinity chromatography procedures for the purification of insulin or HSA. |
| 语种 | 英语 |
| 出版者 | KLUWER ACADEMIC PUBL |
| WOS记录号 | WOS:000072477800013 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/74443]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chase, HA |
| 作者单位 | 1.Univ Cambridge, Dept Chem Engn, Cambridge CB2 3RA, England 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, YH,Chase, HA,Liu, GQ. Selection of affinity ligands for protein purification from proteolytic digests[J]. BIOTECHNOLOGY TECHNIQUES,1998,12(3):245-251. |
| APA | Yang, YH,Chase, HA,&Liu, GQ.(1998).Selection of affinity ligands for protein purification from proteolytic digests.BIOTECHNOLOGY TECHNIQUES,12(3),245-251. |
| MLA | Yang, YH,et al."Selection of affinity ligands for protein purification from proteolytic digests".BIOTECHNOLOGY TECHNIQUES 12.3(1998):245-251. |
入库方式： OAI收割
来源：化学研究所
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