A new method for the preparation of polyacrylamide gradient gel-filled capillaries with low UV detection background
文献类型：期刊论文
| 作者 | Chen, Y |
| 刊名 | TALANTA
 |
| 出版日期 | 1998-08-01 |
| 卷号 | 46期号:4页码:727-734 |
| 关键词 | Low Background Polyacrylamide Gradient Gel-filled Capillaries Capillary Gradient Gel Electrophoresis Polyamino Acids Direct Detection Of Unlabelled Polysaccharides |
| ISSN号 | 0039-9140 |
| 英文摘要 | A simple but reproducible method has been explored for the preparation of polyacrylamide gradient gel-filled capillaries with low UV detection background. The principle is to fill a capillary by plugging it into a wide tube already filled with gradient gelling solutions including a section of buffer. Void-free capillaries can be prepared with a full success for gels below 13%T + 5%C (immobilised completely). For gels between 15%T and 25%T + 5%C, the preparation success rate is 80-95%, depending on the gel immobilisation methods. The resulting capillaries allow the use of any available wave-length for sensitive detection and in the separation of polylysines, a great improvement of their detection sensitivity has been achieved, up to 10(3)-10(4) fold as compared to the common gel-filled capillaries. Unlabelled polysaccharides from the sacculi of Escherichia coli can hence directly be detected at 200 nm. These capillaries can continuously be used for more than 2 months in separating the biological polysaccharides at - 200 V cm(-1) and pH 7.8 while at pH 4.7, they can still be used for more than 400 injections (similar to 1 month) of the polylysines at + 180 V cm(-1). As expected by stacking effect, the gradient gels yield higher efficiency or running speed (1-fold) and even higher UV detection sensitivity (> 2-fold) than the homogeneous gels. (C) 1998 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000074745200032 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/74665]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Y |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Y. A new method for the preparation of polyacrylamide gradient gel-filled capillaries with low UV detection background[J]. TALANTA,1998,46(4):727-734. |
| APA | Chen, Y.(1998).A new method for the preparation of polyacrylamide gradient gel-filled capillaries with low UV detection background.TALANTA,46(4),727-734. |
| MLA | Chen, Y."A new method for the preparation of polyacrylamide gradient gel-filled capillaries with low UV detection background".TALANTA 46.4(1998):727-734. |
入库方式： OAI收割
来源：化学研究所
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