Formulation of matrix solutions for use in matrix-assisted laser desorption ionization of biomolecules
文献类型:期刊论文
作者 | Sze, ETP; Chan, TWD; Wang, G |
刊名 | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
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出版日期 | 1998-02-01 |
卷号 | 9期号:2页码:166-174 |
ISSN号 | 1044-0305 |
英文摘要 | We report a simple method for converting solid matrices into useful matrix solutions for matrix-assisted laser desorption/ionization (MALDI). This method is based on the dissolution of the solid matrix in a liquid support of low volatility such as glycerol. An appropriate solubilizing reagent was added to promote the dissolution of the matrix materials into the Liquid support. Selection of the solubilizing reagent is empirically related to an acid-base relationship, i.e., an acidic solid matrix requires a basic organic compound to form a stable matrix solution in the liquid support and vice versa. A tenfold increase in the solubility can be obtained for many solid matrices when appropriate solubilizing reagents are added into the glycerol support. This solubility enhancement is tentatively attributed to the ion-pair formation in a polar nonvolatile liquid support. Ln addition, the hydrophobicity of the solid matrix seems to play an important role in the efficiency of the resulting matrix solution. By using glycerol as liquid support, a hydrophilic matrix, such as 2,5-dihydroxybenzoic acid (DHB), showed a substantial "peripheral effect," in which good analyte ion signals could only be recorded at the peripheral region of the sample droplet. More hydrophobic matrices, such as alpha-cyano-4-hydroxycinnamic acid (alpha-CCA), exhibit better and more homogeneous responses at different regions of the droplets. The performance of these matrix solutions was evaluated in terms of the durability, reproducibility, sensitivity, high mass capability, and generality. A typical sample droplet can afford more than an hour of repeated sampling with excellent shot-to-shot reproducibility. A low picomole sensitivity was demonstrated using a luteinizing hormone releasing hormone (LHRH) in a Fourier transform ion cyclotron resonance mass spectrometer with a homemade external MALDI ion source. By using a commercial MALDI time-of-flight mass spectrometer, proteins with masses as high as 66,000 Da were successfully analyzed by using these matrix solutions. (C) 1998 American Society for Mass Spectrometry. |
语种 | 英语 |
WOS记录号 | WOS:000071650400008 |
出版者 | ELSEVIER SCIENCE INC |
源URL | [http://ir.iccas.ac.cn/handle/121111/74687] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Chan, TWD |
作者单位 | 1.Chinese Univ Hong Kong, Dept Chem, Shatin, Hong Kong 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
推荐引用方式 GB/T 7714 | Sze, ETP,Chan, TWD,Wang, G. Formulation of matrix solutions for use in matrix-assisted laser desorption ionization of biomolecules[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,1998,9(2):166-174. |
APA | Sze, ETP,Chan, TWD,&Wang, G.(1998).Formulation of matrix solutions for use in matrix-assisted laser desorption ionization of biomolecules.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,9(2),166-174. |
MLA | Sze, ETP,et al."Formulation of matrix solutions for use in matrix-assisted laser desorption ionization of biomolecules".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 9.2(1998):166-174. |
入库方式: OAI收割
来源:化学研究所
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