Gadolinium induces domain and pore formation of human erythrocyte membrane: an atomic force microscopic study
文献类型：期刊论文
| 作者 | Cheng, Y; Liu, MZ; Li, RC; Wang, C; Bai, CL; Wang, K |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
 |
| 出版日期 | 1999-10-15 |
| 卷号 | 1421期号:2页码:249-260 |
| 关键词 | Gadolinium Domain Pore Erythrocyte Membrane Afm |
| ISSN号 | 0005-2736 |
| 英文摘要 | Lanthanide cations bind to human erythrocyte membranes and enhance cell permeability. It was postulated that this effect is due to their likeness with calcium ions, which have been used to induce perforation of cells. However, the nature and mechanism of the perforation are still not clear. In the present work, the change in surface topography of erythrocyte membranes exposed to various gadolinium species was imaged with an atomic force microscope (AFM) in order to get direct evidence of perforation. The images of the whole cell and regions in nanometer scale showed that the normal surface is featured by closely packed nanometer size particles. The AFM images showed that Gd3+ binding to erythrocytes led to domain structure at low concentration and pore formation at higher concentration. The domain structures that appeared after incubation with 1.0 x 10(-6) - 1.0 x 10(-5) mol/l Gd3+ solution for 30 min are featured by the particles aggregated to form ranges and the separations among them enlarged to gorges. With a higher concentration, 2.5 x 10(-5) mol/l Gd3+, the further aggregation developed into crater-shaped 'pores'. By washing with EDTA the 'pores' can be resealed but the domain structure remained. The anionic complex of Gd3+, [Gd(Cit)(2)](3-) of this concentration, can only induce the domain structure formation. The domain and 'pore' structures mediated by Gd3+ concentrations might be responsible for both enhanced permeability and perforation. The mechanism of Gd-induced domain formation and perforation is discussed on the basis of aggregation of membrane proteins and the coexistence of different phases of membrane lipids resulting from Gd3+ binding. (C) 1999 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000083118200004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/75061]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, K |
| 作者单位 | 1.Beijing Med Univ, Natl Res Labs Nat & Biomimet Drugs, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Y,Liu, MZ,Li, RC,et al. Gadolinium induces domain and pore formation of human erythrocyte membrane: an atomic force microscopic study[J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1999,1421(2):249-260. |
| APA | Cheng, Y,Liu, MZ,Li, RC,Wang, C,Bai, CL,&Wang, K.(1999).Gadolinium induces domain and pore formation of human erythrocyte membrane: an atomic force microscopic study.BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES,1421(2),249-260. |
| MLA | Cheng, Y,et al."Gadolinium induces domain and pore formation of human erythrocyte membrane: an atomic force microscopic study".BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES 1421.2(1999):249-260. |
入库方式： OAI收割
来源：化学研究所
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