Effects of training of operant conditioned reflex on free amino acids in hippocampus of rats
文献类型：期刊论文
| 作者 | Shu, HJ; Han, HW; Zhao, R; Chen, Y; Liu, GQ |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 1999-11-01 |
| 卷号 | 20期号:11页码:1703-1707 |
| 关键词 | Operant Conditioned Reflex Hippocampus Learning Amino Acid Analysis |
| ISSN号 | 0251-0790 |
| 英文摘要 | Changes of intracellular levels of free amino acids(FAA) in hippocampus of freely moving rats were measured using intracerebral microdialysis coupled to Orthophthaladehyde (OPA) pre-column derivatization-reversed phase HPLC-fluorescence detection, The analytical method was systematically optimized and the real samples were determined. It was employed to analyze the trace level of free amino acids(FAA) and possible to qualitative and quantitative 18 kinds of amino acids in every 5 mu L microdialysate. The sensitivity and the linear relation between the amounts and the peak areas were satisfied. The recoveries were 82%-109%, The detection limits reached 0. 5-5. 7 pmol for each FAA. A significant decrease of 14 FAA after training of OCR was founded in comparison to basal condition(before training), In fore finds of neurotransmitter amino acids(NAA), excitatory NAA, glutamic acid and aspartic acid increased 19. 12% and 53. 40% respectively than before training, Inhibitory NAA, gamma-aminobutyric (GABA), increased 138. 17% than before training. The results confirm that metabolic increase of FAA in hippocampus can be induced by learning and memory in rats, The intracerebral microdialysis technique thus appears to be a useful tool to monitor changes in physiological activity of learning and memory during ongoing behavior. |
| 语种 | 英语 |
| WOS记录号 | WOS:000083723600010 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/75085]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Han, HW |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shu, HJ,Han, HW,Zhao, R,et al. Effects of training of operant conditioned reflex on free amino acids in hippocampus of rats[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,1999,20(11):1703-1707. |
| APA | Shu, HJ,Han, HW,Zhao, R,Chen, Y,&Liu, GQ.(1999).Effects of training of operant conditioned reflex on free amino acids in hippocampus of rats.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,20(11),1703-1707. |
| MLA | Shu, HJ,et al."Effects of training of operant conditioned reflex on free amino acids in hippocampus of rats".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 20.11(1999):1703-1707. |
入库方式： OAI收割
来源：化学研究所
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