Optimization of preparative conditions for poly-DL-lactide-polyethylene glycol microspheres with entrapped Vibrio Cholera antigens
文献类型：期刊论文
| 作者 | Deng, XM; Li, XH; Yuan, ML; Xiong, CD; Huang, ZT; Jia, WX; Zhang, YH |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 1999-03-29 |
| 卷号 | 58期号:2页码:123-131 |
| 关键词 | Poly-dl-lactide-polyethylene Glycol Vibrio Cholera Antigen Microsphere Loading Efficiency Release Profile |
| ISSN号 | 0168-3659 |
| 英文摘要 | Poly-dl-lactide-polyethylene glycol (PELA) with different contents of polyethylene glycol(PEG) were synthesized and the PEG content was estimated according to the integral height of hydrogen shown in H-1-NMR. PELA microspheres containing V. cholera antigen, outer membrane protein (OMP) were prepared by a water-in-oil-in-water (W/O/W) based on solvent evaporation procedure. Antigen microspheres with smooth surface, suitable size for oral administration (0.5-5 mu m), high loading efficiency (about 60%) and low level of residual solvent (lower than 20ppm) were obtained. Microspheres prepared from PELA with PEG content of about 10% achieved the highest loading efficiency among PELA copolymers and poly-dl-lactide (PLA) homopolymer, which suggested that microspheres size, morphology and the precipitation rate of polymer showed considerable relations with OMP loading efficiency. The regulation of the solvent components of the oil phase contributes to a stable emulsion W/O, and it is concluded that the stable emulsion W/O plays a significant role in improving the protein loading efficiency of obtained microspheres. The addition of stabilizer, such as gelatin and polyvinyl alcohol, into the internal water phase before emulsification produced no significant difference in OMP entrapment and microspheres size. A higher OMP loading efficiency was achieved by adding NaCl or adjusting the pH at the iso-electric point of OMP in the external water phase. It was indicated in vitro that PELA microspheres with smaller size showed larger extent of initial release and higher release rate, whereas microspheres with the diameter of 2.17 mu m showed no apparent burst effect. (C) 1999 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000079277200001 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/75493]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Deng, XM |
| 作者单位 | 1.Chinese Acad Sci, Chengdu Inst Organ Chem, Chengdu 610041, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 3.W China Univ Med Sci, Chengdu 610041, Peoples R China |
| 推荐引用方式 GB/T 7714 | Deng, XM,Li, XH,Yuan, ML,et al. Optimization of preparative conditions for poly-DL-lactide-polyethylene glycol microspheres with entrapped Vibrio Cholera antigens[J]. JOURNAL OF CONTROLLED RELEASE,1999,58(2):123-131. |
| APA | Deng, XM.,Li, XH.,Yuan, ML.,Xiong, CD.,Huang, ZT.,...&Zhang, YH.(1999).Optimization of preparative conditions for poly-DL-lactide-polyethylene glycol microspheres with entrapped Vibrio Cholera antigens.JOURNAL OF CONTROLLED RELEASE,58(2),123-131. |
| MLA | Deng, XM,et al."Optimization of preparative conditions for poly-DL-lactide-polyethylene glycol microspheres with entrapped Vibrio Cholera antigens".JOURNAL OF CONTROLLED RELEASE 58.2(1999):123-131. |
入库方式： OAI收割
来源：化学研究所
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