中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Optimization of preparative conditions for poly-DL-lactide-polyethylene glycol microspheres with entrapped Vibrio Cholera antigens

文献类型:期刊论文

作者Deng, XM; Li, XH; Yuan, ML; Xiong, CD; Huang, ZT; Jia, WX; Zhang, YH
刊名JOURNAL OF CONTROLLED RELEASE
出版日期1999-03-29
卷号58期号:2页码:123-131
关键词Poly-dl-lactide-polyethylene Glycol Vibrio Cholera Antigen Microsphere Loading Efficiency Release Profile
ISSN号0168-3659
英文摘要Poly-dl-lactide-polyethylene glycol (PELA) with different contents of polyethylene glycol(PEG) were synthesized and the PEG content was estimated according to the integral height of hydrogen shown in H-1-NMR. PELA microspheres containing V. cholera antigen, outer membrane protein (OMP) were prepared by a water-in-oil-in-water (W/O/W) based on solvent evaporation procedure. Antigen microspheres with smooth surface, suitable size for oral administration (0.5-5 mu m), high loading efficiency (about 60%) and low level of residual solvent (lower than 20ppm) were obtained. Microspheres prepared from PELA with PEG content of about 10% achieved the highest loading efficiency among PELA copolymers and poly-dl-lactide (PLA) homopolymer, which suggested that microspheres size, morphology and the precipitation rate of polymer showed considerable relations with OMP loading efficiency. The regulation of the solvent components of the oil phase contributes to a stable emulsion W/O, and it is concluded that the stable emulsion W/O plays a significant role in improving the protein loading efficiency of obtained microspheres. The addition of stabilizer, such as gelatin and polyvinyl alcohol, into the internal water phase before emulsification produced no significant difference in OMP entrapment and microspheres size. A higher OMP loading efficiency was achieved by adding NaCl or adjusting the pH at the iso-electric point of OMP in the external water phase. It was indicated in vitro that PELA microspheres with smaller size showed larger extent of initial release and higher release rate, whereas microspheres with the diameter of 2.17 mu m showed no apparent burst effect. (C) 1999 Elsevier Science B.V. All rights reserved.
语种英语
WOS记录号WOS:000079277200001
出版者ELSEVIER SCIENCE BV
源URL[http://ir.iccas.ac.cn/handle/121111/75493]  
专题中国科学院化学研究所
通讯作者Deng, XM
作者单位1.Chinese Acad Sci, Chengdu Inst Organ Chem, Chengdu 610041, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China
3.W China Univ Med Sci, Chengdu 610041, Peoples R China
推荐引用方式
GB/T 7714
Deng, XM,Li, XH,Yuan, ML,et al. Optimization of preparative conditions for poly-DL-lactide-polyethylene glycol microspheres with entrapped Vibrio Cholera antigens[J]. JOURNAL OF CONTROLLED RELEASE,1999,58(2):123-131.
APA Deng, XM.,Li, XH.,Yuan, ML.,Xiong, CD.,Huang, ZT.,...&Zhang, YH.(1999).Optimization of preparative conditions for poly-DL-lactide-polyethylene glycol microspheres with entrapped Vibrio Cholera antigens.JOURNAL OF CONTROLLED RELEASE,58(2),123-131.
MLA Deng, XM,et al."Optimization of preparative conditions for poly-DL-lactide-polyethylene glycol microspheres with entrapped Vibrio Cholera antigens".JOURNAL OF CONTROLLED RELEASE 58.2(1999):123-131.

入库方式: OAI收割

来源:化学研究所

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