pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films
文献类型：期刊论文
| 作者 | Jussila, Tapio1; Li, Meiling2; Tkachenko, Nikolai V.1; Parkkinen, Sinikka3; Li, Baofang2; Jiang, Long2; Lemmetyinen, Helge1 |
| 刊名 | INTERNATIONAL JOURNAL OF PHOTOENERGY
 |
| 出版日期 | 2000 |
| 卷号 | 2页码:41-45 |
| ISSN号 | 1110-662X |
| 英文摘要 | Bacteriorhodopsin (bR) multilayer films were successfully prepared by alternating depositions of cationic poly(diallyldimethylammonium chloride) (PDAC) polyelectrolyte and purple membrane (PM). The advantage of the method when compared to other thin film techniques is its simplicity to prepare large self-assembled films. The orientations of the bR molecules in the films were studied by a transient photovoltage method. The polarity of the photoresponse signals showed that in basic conditions the cytoplasmic side of PM was adsorbed onto the polyelectrolyte layer, whereas in acidic conditions the extracellular side was adsorbed onto the polyelectrolyte layer. The photocycle of the bR in the films was studied by using the conventional flash photolysis method. |
| 语种 | 英语 |
| WOS记录号 | WOS:000208867600006 |
| 出版者 | HINDAWI PUBLISHING CORPORATION |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/75611]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jussila, Tapio |
| 作者单位 | 1.Tampere Univ Technol, Inst Mat Chem, FIN-33101 Tampere, Finland 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Lab Colloid & Interface Sci, Beijing 100101, Peoples R China 3.Univ Joensuu, Dept Biol, FIN-80101 Joensuu, Finland |
| 推荐引用方式 GB/T 7714 | Jussila, Tapio,Li, Meiling,Tkachenko, Nikolai V.,et al. pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films[J]. INTERNATIONAL JOURNAL OF PHOTOENERGY,2000,2:41-45. |
| APA | Jussila, Tapio.,Li, Meiling.,Tkachenko, Nikolai V..,Parkkinen, Sinikka.,Li, Baofang.,...&Lemmetyinen, Helge.(2000).pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films.INTERNATIONAL JOURNAL OF PHOTOENERGY,2,41-45. |
| MLA | Jussila, Tapio,et al."pH dependence of the protein orientation in self-assembled bacteriorhodopsin/polycation multilayer films".INTERNATIONAL JOURNAL OF PHOTOENERGY 2(2000):41-45. |
入库方式： OAI收割
来源：化学研究所
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