Affinity chromatography based on the oligohistines as the ligands
文献类型：期刊论文
| 作者 | Yu, X; Zhao, R; Fang, M; Xiong, SX; Su, TS; Liu, GQ |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2000-04-01 |
| 卷号 | 21期号:4页码:532-534 |
| 关键词 | Igg Oligohistine Hpac Dissociation Constant Synthesis Of Peptide In Solid Phase |
| ISSN号 | 0251-0790 |
| 英文摘要 | A series of affinity media were synthesized by using the oligohistines, His-Gly, (His)(3)-Gly and (His)(5)-Gly as the ligands and the home-made monosized, non-porous copolymer (CL-PGMA) beads containing active epoxide groups as the support. The affinity chromatographic behavior between IgG and the oligohistines was investigated at pH 3. 0, 4. 5, 7. 0 and 10. 0. It showed the specific interaction at pH 4. 5. The dissociation constants of IgG and oligohistine media (1H, 3H, 5H) were 3. 037 X 10(-6), 2. 459 X 10(-6) and 1. 905 X 10(-6) L/mol, respectively. The dissociation constants decreased with increasing the number of histine-residue in oligopeptide chains. |
| 语种 | 英语 |
| WOS记录号 | WOS:000086574700009 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/75869]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, R |
| 作者单位 | Chinese Acad Sci, Ctr Mol Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, X,Zhao, R,Fang, M,et al. Affinity chromatography based on the oligohistines as the ligands[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2000,21(4):532-534. |
| APA | Yu, X,Zhao, R,Fang, M,Xiong, SX,Su, TS,&Liu, GQ.(2000).Affinity chromatography based on the oligohistines as the ligands.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,21(4),532-534. |
| MLA | Yu, X,et al."Affinity chromatography based on the oligohistines as the ligands".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 21.4(2000):532-534. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。