Effect of CO2 and CHF3 on the solubilization of protein in reverse micelles
文献类型:期刊论文
作者 | Zhang, HF; Han, BX; Yang, GY; Yan, HK |
刊名 | JOURNAL OF COLLOID AND INTERFACE SCIENCE
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出版日期 | 2000-12-15 |
卷号 | 232期号:2页码:269-272 |
关键词 | Compressed Gases Reverse Micelles Protein Precipitation Co2 Chf3 |
ISSN号 | 0021-9797 |
DOI | 10.1006/jcis.2000.7214 |
英文摘要 | The effect of dissolved CO2 and CHF3 on the precipitation of bovine serum albumin (BSA) from reverse micelles of sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in 2,2,4-trimethylpentane (isooctane) has been studied at 308.15 K. It was found that BSA can be precipitated completely by CO2 while the AOT and water remain in the iso-octane continuous phase. This opens up a possibility for recovery of protein solids directly from reverse micellar solutions. CHF3 is also an effective agent for precipitation of BSA in the reverse micelles when the expansion coefficient of CHF3 exceeds 0.4. However, the condition required for complete removal of the BSA using CHF3 results in some precipitation of AOT and water from the solution. (C) 2000 Academic Press. |
语种 | 英语 |
WOS记录号 | WOS:000165866300008 |
出版者 | ACADEMIC PRESS INC |
源URL | [http://ir.iccas.ac.cn/handle/121111/76103] ![]() |
专题 | 中国科学院化学研究所 |
通讯作者 | Han, BX |
作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, HF,Han, BX,Yang, GY,et al. Effect of CO2 and CHF3 on the solubilization of protein in reverse micelles[J]. JOURNAL OF COLLOID AND INTERFACE SCIENCE,2000,232(2):269-272. |
APA | Zhang, HF,Han, BX,Yang, GY,&Yan, HK.(2000).Effect of CO2 and CHF3 on the solubilization of protein in reverse micelles.JOURNAL OF COLLOID AND INTERFACE SCIENCE,232(2),269-272. |
MLA | Zhang, HF,et al."Effect of CO2 and CHF3 on the solubilization of protein in reverse micelles".JOURNAL OF COLLOID AND INTERFACE SCIENCE 232.2(2000):269-272. |
入库方式: OAI收割
来源:化学研究所
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