Monosized non-porous poly(glycidyl methacrylate) beads as the support for high performance affinity chromatography
文献类型：期刊论文
| 作者 | Yu, X; Zhao, R; Fang, M; Xiong, SX; Guo, YH; Su, TS; Liu, GQ |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2000-05-20 |
| 卷号 | 28期号:5页码:544-548 |
| 关键词 | High Performance Affinity Chromatography Monosized Non-porous Poly(Glycidyl Methacrylate) Beads Trypsin Inhibitor |
| ISSN号 | 0253-3820 |
| 英文摘要 | A new synthetic support, monosized, non-porous, cross-linked bead CL-poly(glycidyl methacrylate) (CL-PGMA) containing active epoxide groups was synthesized and used as the support of affinity chromatography. The concentration of the epoxide groups on the surface of CL-PGMA beads was measured and the non-specific adsorption to protein was characterized. Trypsin inhibitor was chosen as the ligand. The trypsin was purified 2.5 times by using the trypsin inhibitor immobilized CL-PGMA affinity column. The chromatographic characterization was shown that the CL-PGMA bead was very suitable for the high performance affinity chromatography. The effective elimination of non-specific adsorption was carried out by adding 1% acetonitrile in mobile phase. The recovery of human serum albumin was more than 99%. |
| 语种 | 英语 |
| WOS记录号 | WOS:000087271600004 |
| 出版者 | FENXI HUAXUE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/76123]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, R |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, X,Zhao, R,Fang, M,et al. Monosized non-porous poly(glycidyl methacrylate) beads as the support for high performance affinity chromatography[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2000,28(5):544-548. |
| APA | Yu, X.,Zhao, R.,Fang, M.,Xiong, SX.,Guo, YH.,...&Liu, GQ.(2000).Monosized non-porous poly(glycidyl methacrylate) beads as the support for high performance affinity chromatography.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,28(5),544-548. |
| MLA | Yu, X,et al."Monosized non-porous poly(glycidyl methacrylate) beads as the support for high performance affinity chromatography".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 28.5(2000):544-548. |
入库方式： OAI收割
来源：化学研究所
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