Stability investigation of the mixed DPPC/protein monolayer at the air-water interface
文献类型：期刊论文
| 作者 | Zhang, HJ; Wang, XL; Cui, GC; Li, JB |
| 刊名 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| 出版日期 | 2000-12-15 |
| 卷号 | 175期号:1-2页码:77-82 |
| 关键词 | Brewster Angle Microscopy Subphase Exchange Mixed L-alpha-dipalmitoylphosphatidylcholine/beta-lactoglobulin Monolayer |
| ISSN号 | 0927-7757 |
| 英文摘要 | A subphase exchange method has been used to investigate the stability of the mixed L-alpha-dipalmitoylphosphatidylcholine (DPPC)/beta-lactoglobulin monolayer formed via the protein adsorption into the spread lipid layer at the air-water interface by Brewster angle microscopy (BAM). The BAM images demonstrated that the adsorbed protein might compress the DPPC monolayer to enter a phase transition early close to a condensed phase. When protein concentration is very high the penetration of the protein is rather fast, and a larger domain was preferentially formed without compressing the DPPC monolayer. After the mixed DPPC/beta-lactoglobulin monolayer was formed, the protein solution in subphase was totally washed out by pumping in buffer water for a long time. In the fluid phase and coexistence region of DPPC monolayer with penetrated protein layer the domains have been recorded by BAM images for comparing the variation before and after the exchange of subphase. The experimental results revealed that there was not significant change of the domains in size and shape. It indicates that the adsorbed protein has a strong interaction with DPPC and is more likely to remain at the interface. A relatively stable mixed lipid-protein monolayer can be constructed by this way. Hydrophobic and electrostatic interactions between DPPC and beta-lactoglobulin as well as the conformation change of beta-lactoglobulin at air-water interface have been taken into account to stabilize the mixed layers, (C) 2000 published by Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000089562300010 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/76145]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, JB |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Int Joint Lab,Inst Photog Chem, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Max Planck Inst Colloid & Interface Sci, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, HJ,Wang, XL,Cui, GC,et al. Stability investigation of the mixed DPPC/protein monolayer at the air-water interface[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2000,175(1-2):77-82. |
| APA | Zhang, HJ,Wang, XL,Cui, GC,&Li, JB.(2000).Stability investigation of the mixed DPPC/protein monolayer at the air-water interface.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,175(1-2),77-82. |
| MLA | Zhang, HJ,et al."Stability investigation of the mixed DPPC/protein monolayer at the air-water interface".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 175.1-2(2000):77-82. |
入库方式： OAI收割
来源：化学研究所
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