Photosensitized formation of singlet oxygen by phycobiliproteins in neutral aqueous solutions
文献类型：期刊论文
| 作者 | Zhang, SP; Zhao, JQ; Jiang, LJ |
| 刊名 | FREE RADICAL RESEARCH
 |
| 出版日期 | 2000 |
| 卷号 | 33期号:5页码:489-496 |
| 关键词 | Phycobiliprotein Singlet Oxygen Photobleaching |
| ISSN号 | 1071-5762 |
| 英文摘要 | Phycobiliproteins (PBPs) are a type of promising sensitizers for photodynamic therapy (PDT). Upon irradiation (lambda >500nm) of an oxygen-saturated aqueous solution of phycobiliproteins, particularly, C-phycocyanin (C-PC), allophycocyanin (APC) or R-phycoerythrin (R-PE), the formation of singlet oxygen (O-1(2)) was detected by using imidazole in the presence of p-nitrosodimethylaniline (RNO). The bleaching of RNO caused by the presence of imidazole in our system showed typical concentration dependence with a maximum at about 8mM imidazole, which is in agreement with the formation of O-1(2) in addition, the generation of O-1(2) was verified further in the presence of D2O and specific singlet oxygen quencher - 1,4-diazabicyclo [2,2,2] octane (DABCO) and sodium azide (NaN3). Our experimental results indicated that APC possesses high ability to generate reactive oxygen species and the relative quantum yields of photogeneration of O-1(2) by PBPs are as follows: APC > C-PC > R-PE. |
| 语种 | 英语 |
| WOS记录号 | WOS:000166441800005 |
| 出版者 | HARWOOD ACAD PUBL GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/76441]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, JQ |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, SP,Zhao, JQ,Jiang, LJ. Photosensitized formation of singlet oxygen by phycobiliproteins in neutral aqueous solutions[J]. FREE RADICAL RESEARCH,2000,33(5):489-496. |
| APA | Zhang, SP,Zhao, JQ,&Jiang, LJ.(2000).Photosensitized formation of singlet oxygen by phycobiliproteins in neutral aqueous solutions.FREE RADICAL RESEARCH,33(5),489-496. |
| MLA | Zhang, SP,et al."Photosensitized formation of singlet oxygen by phycobiliproteins in neutral aqueous solutions".FREE RADICAL RESEARCH 33.5(2000):489-496. |
入库方式： OAI收割
来源：化学研究所
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