Effect of solution pH and composition on horse liver alcohol dehydrogenase thermostability
文献类型：期刊论文
| 作者 | Wang, BN; Han, BX; Tan, F |
| 刊名 | JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
 |
| 出版日期 | 2000 |
| 卷号 | 62期号:1页码:317-324 |
| 关键词 | Alcohol Dehydrogenase Differential Scanning Calorimetry Heat Denaturation Thermostability |
| ISSN号 | 1418-2874 |
| 英文摘要 | The influence of solution composition (pH, salts, and chelant) on the thermostability of horse liver alcohol dehydrogenase was studied by differential scanning calorimetry (DSC) in the pH range from 7.51 to 9.50 and showing the enzyme catalytic activity. The experiments demonstrated that the effect of increasing pH on the heat denaturation temperature of the enzyme was slight, but the denaturation enthalpy was considerably increased, indicating the enzyme conformation alteration by changing pH and the presence of enthalpy-entropy compensation. The effect of ionic strength on thermostability was not noticeable, i.e., the electrostatic interactions were not a dominant factor for the thermostability. The anions Cl- and SCN- imposed diverse influence upon the enzyme thermostability, and SCN- can reduce the thermostability considerably. The chelant 1,10-phenanthroline, which can reversibly bind together with the zinc ions functioning the catalytic action in the enzyme molecules, increases the thermostability considerably. The hydration of the enzyme plays an important role to the thermostability. |
| 语种 | 英语 |
| WOS记录号 | WOS:000089986600030 |
| 出版者 | KLUWER ACADEMIC PUBL |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/76567]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, BN |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, BN,Han, BX,Tan, F. Effect of solution pH and composition on horse liver alcohol dehydrogenase thermostability[J]. JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY,2000,62(1):317-324. |
| APA | Wang, BN,Han, BX,&Tan, F.(2000).Effect of solution pH and composition on horse liver alcohol dehydrogenase thermostability.JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY,62(1),317-324. |
| MLA | Wang, BN,et al."Effect of solution pH and composition on horse liver alcohol dehydrogenase thermostability".JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY 62.1(2000):317-324. |
入库方式： OAI收割
来源：化学研究所
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