High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)(2)(dppx)(2+)
文献类型：期刊论文
| 作者 | Ling, LS; He, ZK; Song, GW; Zeng, YE; Wang, C; Bai, CL; Chen, XD; Shen, P |
| 刊名 | ANALYTICA CHIMICA ACTA
 |
| 出版日期 | 2001-06-12 |
| 卷号 | 436期号:2页码:207-214 |
| 关键词 | Ru(Phen)(2)(Dppx)(2+) Dna Fluorimetry Molecular "light Switch" |
| ISSN号 | 0003-2670 |
| 英文摘要 | A novel fluorimetric method is developed for selective determination of DNA with the molecular "light switch" complex of Ru(phen)(2)(dppx)(2+). The maximal fluorescence intensity is produced in the pH range of 9.3-11.5, with the maximum excitation and emission wavelength of 464 and 594 nm, respectively. Under the optimum conditions, the fluorescence intensity was in proportion to the concentration of DNA. The linear range for calfthymus DNA, salmon sperm DNA and herring sperm DNA is 0-0.2 mug/ml. The limits of detection for calfthymus DNA, salmon sperm DNA and herring sperm DNA are 0.3, 0.24 and 0.74 ng/ml, respectively. And the proposed method was successfully used to determine DNA contents in real sample, which was dealt with RNase before the determination. The mechanism of the fluorescence sensitization is also studied. We also found that Ru(phen)(2)(dppx)(2+) preferalls intercalates into AT base pairs to GC base pairs. (C) 2001 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000169276200004 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/76929]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ling, LS |
| 作者单位 | 1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China 3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ling, LS,He, ZK,Song, GW,et al. High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)(2)(dppx)(2+)[J]. ANALYTICA CHIMICA ACTA,2001,436(2):207-214. |
| APA | Ling, LS.,He, ZK.,Song, GW.,Zeng, YE.,Wang, C.,...&Shen, P.(2001).High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)(2)(dppx)(2+).ANALYTICA CHIMICA ACTA,436(2),207-214. |
| MLA | Ling, LS,et al."High sensitive determination of DNA by use of molecular "light switch" complex of Ru(phen)(2)(dppx)(2+)".ANALYTICA CHIMICA ACTA 436.2(2001):207-214. |
入库方式： OAI收割
来源：化学研究所
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