Determination of carbohydrates as their p-sulfophenylhydrazones by capillary zone electrophoresis
文献类型：期刊论文
| 作者 | Wang, XY; Chen, Y |
| 刊名 | CARBOHYDRATE RESEARCH
 |
| 出版日期 | 2001-05-18 |
| 卷号 | 332期号:2页码:191-196 |
| 关键词 | P-hydrazinobenzenesulfonic Acid Carbohydrate Capillary Electrophoresis Precolumn Derivatization |
| ISSN号 | 0008-6215 |
| 英文摘要 | p-Hydrazinobenzenesulfonic acid was explored as an ultraviolet labeling reagent for capillary electrophoresis of mono-, di- and trisaccharides. The labeling reaction that produces p-sulfophenylhydrazines took less than 8 min, and introduced both chromphore and charged groups into the carbohydrate molecules. The derivatives of nine mono- and disaccharides were completely separated in 9 min using a 100 mM berate buffer at pH 10.24. On-column UV detection at 200 nm allowed the detection of glucose with a mass detection limit of 17.6 fmol or a concentration limit of 3.6 muM. Reproducible quantification of carbohydrates was achieved in the concentration range of 0.1-9.1 mM in reaction solution. The method was applied successfully to determine the monosaccharide composition of laminaran. (C) 2001 Elsevier Science Ltd. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000168884400009 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/76983]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Chen, Y |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Grp 205, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, XY,Chen, Y. Determination of carbohydrates as their p-sulfophenylhydrazones by capillary zone electrophoresis[J]. CARBOHYDRATE RESEARCH,2001,332(2):191-196. |
| APA | Wang, XY,&Chen, Y.(2001).Determination of carbohydrates as their p-sulfophenylhydrazones by capillary zone electrophoresis.CARBOHYDRATE RESEARCH,332(2),191-196. |
| MLA | Wang, XY,et al."Determination of carbohydrates as their p-sulfophenylhydrazones by capillary zone electrophoresis".CARBOHYDRATE RESEARCH 332.2(2001):191-196. |
入库方式： OAI收割
来源：化学研究所
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