Speciation analysis of serum copper by ultrafiltration combined with graphite furnace atomic absorption spectrometry
文献类型：期刊论文
| 作者 | Wang, ZH; Ma, HM; Ma, QL; Liang, SC |
| 刊名 | CHINESE JOURNAL OF CHEMISTRY
 |
| 出版日期 | 2001-02-01 |
| 卷号 | 19期号:2页码:143-146 |
| 关键词 | Ultrafiltration Speciation Analysis Serum Copper Graphite Furnace Atomic Absorption Spectrometry |
| ISSN号 | 1001-604X |
| 英文摘要 | Ultrafiltration combined with graphite furnace atomic absorption spectrometry (GFAAS) was used to study protein binding and speciation of copper in human serum. Ultrafiltration was carried out using a cell unit with ultrafiltration membranes having a nominal cut-off of 10,000 Dalton. The effects of various experimental factors including the kind and concentration of electrolyte, sample storage, pH, pressure and the preconditioning of the membranes on the speciation analysis of serum copper by ultrafiltration were examined. It was observed that 4.5 +/- 2.3% of the total copper in serum was ultrafiltrable and this value did not seem to be influenced by the total serum elemental concentration, the pH (6.5 - 10) and the pressure (I 1.5 kg/cm(2)). The preconditioning of the ultrafiltration system with 0.1 mol/L calcium nitrate can overcome the adsorption loss of copper effectively, and the addition of tris-HCl solution (pH 7.4) to serum accelerates the ultrafiltration. The present method was proved to be suitable for speciation analysis for its simplicity, rapidity, small sample requirement and easy control. The results obtained with the method are accurate and reliable. |
| 语种 | 英语 |
| WOS记录号 | WOS:000166921000004 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/77333]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, ZH |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, ZH,Ma, HM,Ma, QL,et al. Speciation analysis of serum copper by ultrafiltration combined with graphite furnace atomic absorption spectrometry[J]. CHINESE JOURNAL OF CHEMISTRY,2001,19(2):143-146. |
| APA | Wang, ZH,Ma, HM,Ma, QL,&Liang, SC.(2001).Speciation analysis of serum copper by ultrafiltration combined with graphite furnace atomic absorption spectrometry.CHINESE JOURNAL OF CHEMISTRY,19(2),143-146. |
| MLA | Wang, ZH,et al."Speciation analysis of serum copper by ultrafiltration combined with graphite furnace atomic absorption spectrometry".CHINESE JOURNAL OF CHEMISTRY 19.2(2001):143-146. |
入库方式： OAI收割
来源：化学研究所
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