Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis
文献类型：期刊论文
| 作者 | Zhang, DM; Zhang, JM; Ma, WY; Chen, DY; Han, HW; Shu, HJ; Liu, GQ |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B
 |
| 出版日期 | 2001-07-15 |
| 卷号 | 758期号:2页码:277-282 |
| ISSN号 | 0378-4347 |
| 关键词 | Amino Acid Neurotransmitters |
| 英文摘要 | A simple but effective coupling of microdialysis and capillary electrophoresis with laser induced fluorescence detection technique was applied to analysis of amino acid neurotransmitters in the hypothalamus of rats after acute exhausting exercise. The separation of amino acids was achieved using an uncoated fused-silica capillary (57 cm x 75 mum I.D.) with a buffer of 10 mM disodium tetraborate at pH 10 and an applied voltage of 12.5 kV. The detection limit was 10(-10) M for each amino acid. It is sufficiently sensitive and rapid for the determination of amino acids in a 5-mul Microdialysate. In comparison to pre-exercise, a significant increase in the levels of six hypothalamic amino acids (arginine, glycine, lysine, glutamic acid, alanine, gamma -amino-n-butyric acid) was found after exercise. These results demonstrate that the increase of metabolic amino acids in the hypothalamus of rats can be induced by exhausting exercise and suggests that amino acid neurotransmitters may play functional roles in the central effects of exercise. (C) 2001 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000169712400017 |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/77365]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, WY |
| 作者单位 | 1.Tsing Hua Univ, Dept Modern Appl Phys, Ctr Atom & Mol Sci, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, DM,Zhang, JM,Ma, WY,et al. Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis[J]. JOURNAL OF CHROMATOGRAPHY B,2001,758(2):277-282. |
| APA | Zhang, DM.,Zhang, JM.,Ma, WY.,Chen, DY.,Han, HW.,...&Liu, GQ.(2001).Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis.JOURNAL OF CHROMATOGRAPHY B,758(2),277-282. |
| MLA | Zhang, DM,et al."Analysis of trace amino acid neurotransmitters in hypothalamus of rats after exhausting exercise using microdialysis".JOURNAL OF CHROMATOGRAPHY B 758.2(2001):277-282. |
入库方式： OAI收割
来源：化学研究所
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