Transient absorption and photovoltage study of' self-assembled bacteriorhodopsin/polycation multilayer films
文献类型：期刊论文
| 作者 | Jussila, T; Li, ML; Tkachenko, NV; Parkkinen, S; Li, BF; Jiang, L; Lemmetyinen, H |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2002-06-01 |
| 卷号 | 17期号:6-7页码:509-515 |
| 关键词 | Bacteriorhodopsin Polyelectrolyte Multilayer Film Self-assembly |
| ISSN号 | 0956-5663 |
| 英文摘要 | A series of organized (PDAC/PM)(n) (poly(diallyldimethylammonium chloride)/purple membrane) multilayer films were prepared by alternate adsorptions of positively charged PDAC polyelectrolyte and negatively charged purple membrane (PM). The kinetics of the photocycle of bacteriorhodopsin (bR) in PM was studied by flash photolysis and transient photovoltage methods. Although the orientation of the adsorbed bR depends on the pH of the PM suspension, the kinetics of the photo-induced reaction cycle in dehydrated films is independent of the deposition pH. In dry (PDAC/PM)(n) films the decay of the M intermediate to the initial bR state is multiexponential and delayed to several minutes for both orientations. A simultaneous two-exponential decay in millisecond time domain was observed at red wavelengths. The source of the red-shifted absorption is suggested to be the C-610 intermediate of the cis photocycle of bR. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000175329400009 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/78919]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jussila, T |
| 作者单位 | 1.Tampere Univ Technol, Inst Mat Chem, FIN-33101 Tampere, Finland 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Lab Colloid & Interface Sci, Beijing 100101, Peoples R China 3.Univ Joensuu, Dept Biol, FIN-80101 Joensuu, Finland |
| 推荐引用方式 GB/T 7714 | Jussila, T,Li, ML,Tkachenko, NV,et al. Transient absorption and photovoltage study of' self-assembled bacteriorhodopsin/polycation multilayer films[J]. BIOSENSORS & BIOELECTRONICS,2002,17(6-7):509-515. |
| APA | Jussila, T.,Li, ML.,Tkachenko, NV.,Parkkinen, S.,Li, BF.,...&Lemmetyinen, H.(2002).Transient absorption and photovoltage study of' self-assembled bacteriorhodopsin/polycation multilayer films.BIOSENSORS & BIOELECTRONICS,17(6-7),509-515. |
| MLA | Jussila, T,et al."Transient absorption and photovoltage study of' self-assembled bacteriorhodopsin/polycation multilayer films".BIOSENSORS & BIOELECTRONICS 17.6-7(2002):509-515. |
入库方式： OAI收割
来源：化学研究所
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