Difference in enzyme activity and conformation of glucose oxidase before and after purification
文献类型：期刊论文
| 作者 | Dai, GL; Li, JR; Jiang, L |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2002-04-01 |
| 卷号 | 24期号:3-4页码:171-176 |
| 关键词 | God Purification Surface Pressure-area Isotherms Surface Pressure-time Isotherms Octadecylamine |
| ISSN号 | 0927-7765 |
| 英文摘要 | Enzyme activity of commercial glucose oxidase was enhanced after purification through a strong anionic exchange resin. In order to get a better insight into this phenomenon, surface pressure-area (pi-A) isotherms and surface pressure-time (pi-t) isotherms was used to study the interaction and the absorption at different pH values of the subphases between octadecylamine and glucose oxidase purified by a styrene system quaternary ammonium type strongly basic anionic exchange resin. Circular dichroism (CD), electrophoresis and enzyme activity measurements were conducted to study these phenomena. A preliminary hypothesis has been suggested to explain why the enzyme activity of purified glucose oxidase was higher than that of the commercial one. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000174555700001 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/79215]  |
| 专题 | 中国科学院化学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, GL,Li, JR,Jiang, L. Difference in enzyme activity and conformation of glucose oxidase before and after purification[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2002,24(3-4):171-176. |
| APA | Dai, GL,Li, JR,&Jiang, L.(2002).Difference in enzyme activity and conformation of glucose oxidase before and after purification.COLLOIDS AND SURFACES B-BIOINTERFACES,24(3-4),171-176. |
| MLA | Dai, GL,et al."Difference in enzyme activity and conformation of glucose oxidase before and after purification".COLLOIDS AND SURFACES B-BIOINTERFACES 24.3-4(2002):171-176. |
入库方式： OAI收割
来源：化学研究所
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