Preparation and characteristics of high performance affinity chromatography medium used for the separation of antithrombin III
文献类型：期刊论文
| 作者 | Zhao, YX; Shangguan, DH; Zhao, R; Liu, GQ |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2002-02-01 |
| 卷号 | 23期号:2页码:203-206 |
| 关键词 | High Performance Affinity Chromatography Heparin Antithrombin Iii |
| ISSN号 | 0251-0790 |
| 英文摘要 | A high performance affinity chromatography medium used for separation of antithrombin III was prepared. Monodisperse poly (glycidyl methacrylate) beads (PGMA) were employed as the affinity support. Heparin was linked covalently to amino-PGMA-beads, which was prepared by amination of PGMA. The resultant affinity media present a minimal non-specific interaction with proteins and the protein recovery was 96. 9% and 94. 4% for antithrombin III and human serum albumin, respectively. Its affinity capacity for antithrombin was 1. 2 mg/g beads. The apparent dissociation constant between antithrombin II and immobilized heparin was 1. 96 X 10(-5) mol/L which was evaluated by front chromatography. The affinity medium was used to separate antithrombin E from human plasma. |
| 语种 | 英语 |
| WOS记录号 | WOS:000174163300010 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/79395]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, R |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, YX,Shangguan, DH,Zhao, R,et al. Preparation and characteristics of high performance affinity chromatography medium used for the separation of antithrombin III[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(2):203-206. |
| APA | Zhao, YX,Shangguan, DH,Zhao, R,&Liu, GQ.(2002).Preparation and characteristics of high performance affinity chromatography medium used for the separation of antithrombin III.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,23(2),203-206. |
| MLA | Zhao, YX,et al."Preparation and characteristics of high performance affinity chromatography medium used for the separation of antithrombin III".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 23.2(2002):203-206. |
入库方式： OAI收割
来源：化学研究所
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