Solid phase derivatization of amino acids and peptides on alkaline silica gel support
文献类型：期刊论文
| 作者 | Shangguan, DH; Zhao, YX; Han, HW; Zhao, R; Liu, GQ |
| 刊名 | CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
 |
| 出版日期 | 2002-03-01 |
| 卷号 | 23期号:3页码:385-387 |
| 关键词 | Solid Phase Fluorescent Derivatization Fmoc-cl Alkaline Silica Gel Amino Acids Peptides |
| ISSN号 | 0251-0790 |
| 英文摘要 | A solid phase derivatization method of amino acids and peptides was described. Samples were added on alkaline silica gel cartridge and dried. The derivatization was carried out by passing the derivatization regent dissolved in anhydrous solvent through the cartridge slowly, then the cartridge was washed with a selected solvent, finally, the derivatives were eluted. The eluates were separated and detected by means of HPLC with fluorescence detector. Three derivatization reagents, 9-fluorenylmethyl chloroformate (FMOC-Cl), 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and fluorescein isothiocyanate (FITC) were investigated with this method. The results showed that the method was suitable for derivatization of amino acids and some of peptides with FMOC-Cl because of its highly reacting activity and low polarity, the side-products of the FMOC-Cl with water were greatly decreased, the excess FMOC-Cl was eliminated completely, and the silica got support did not significantly affect the reaction rate and yield for amino acids. NBD-Cl was not suitable for this method because of the low yield of derivatization. FITC was also not suitable for this method because of its strong polarity. |
| 语种 | 英语 |
| WOS记录号 | WOS:000175465700012 |
| 出版者 | HIGHER EDUCATION PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/79405]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, GQ |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shangguan, DH,Zhao, YX,Han, HW,et al. Solid phase derivatization of amino acids and peptides on alkaline silica gel support[J]. CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,2002,23(3):385-387. |
| APA | Shangguan, DH,Zhao, YX,Han, HW,Zhao, R,&Liu, GQ.(2002).Solid phase derivatization of amino acids and peptides on alkaline silica gel support.CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE,23(3),385-387. |
| MLA | Shangguan, DH,et al."Solid phase derivatization of amino acids and peptides on alkaline silica gel support".CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE 23.3(2002):385-387. |
入库方式： OAI收割
来源：化学研究所
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