A new type of capillary column for open-tubular electrochromatography
文献类型：期刊论文
| 作者 | Zhao, YX; Zhao, R; Shangguan, DH; Liu, GQ |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2002-09-01 |
| 卷号 | 23期号:17页码:2990-2995 |
| 关键词 | Open-tubular Capillary Electrochromatography Peptides Sol-gel Technology |
| ISSN号 | 0173-0835 |
| 英文摘要 | A new type of open-tubular C-18 ester-bonded electrochromatographic column was prepared with sol-gel technology, followed by an on-column octadecyl silylation reaction, Glycidoxypropyltrimethoxysilane, a widely used and important silane agent, was used as the sol-gel precursor to form a thin coating layer on the wall of the fused-silica capillary. The C-18 groups were introduced into the coating layer by on-column esterification reaction with stearic acid. The electrochromatography behavior of the column was evaluated in terms of the separation of peptides. A high efficiency of 4.8 x 10(5) plates/m was achieved for a basic pentapeptide using the C-18 ester-bonded column. In comparison with bare capillaries and glycidoxypropyltrimethoxysilane sol-gel-coated capillaries, the C-18 ester-bonded column showed the best separation of a mixture of seven pentapeptides under identical conditions of buffer, pH, and applied voltage. |
| 语种 | 英语 |
| WOS记录号 | WOS:000178154100021 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/79441]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, GQ |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, YX,Zhao, R,Shangguan, DH,et al. A new type of capillary column for open-tubular electrochromatography[J]. ELECTROPHORESIS,2002,23(17):2990-2995. |
| APA | Zhao, YX,Zhao, R,Shangguan, DH,&Liu, GQ.(2002).A new type of capillary column for open-tubular electrochromatography.ELECTROPHORESIS,23(17),2990-2995. |
| MLA | Zhao, YX,et al."A new type of capillary column for open-tubular electrochromatography".ELECTROPHORESIS 23.17(2002):2990-2995. |
入库方式： OAI收割
来源：化学研究所
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