Separation of four basic proteins in the mixture by capillary electrophoresis with a new chemical modification column
文献类型：期刊论文
| 作者 | Fang, M; Zhao, R; Shen, L; Su, TS; Liu, GQ |
| 刊名 | ANALYTICAL LETTERS
 |
| 出版日期 | 2002 |
| 卷号 | 35期号:2页码:397-411 |
| 关键词 | Capillary Electrophoresis Proteins Separation Lysozyme Cytochrome c Ribonuclease a Alpha-chymotrypsinogen |
| ISSN号 | 0003-2719 |
| 英文摘要 | Chemical modification of internal wall of capillary by in situ polymerization method using a reagent containing two function groups, methacryloxypropyl trimethyoxysilane (KH-570) as coupling agent and glycidyl methacrylate (GMA) as coating material in the presence of initiator was carried out. After hydrolysis of polyglycidyl methacrylate (PGMA), the novel modified capillary column was prepared. The electrophoretic evaluation of the modified column was performed. The results showed that electroosmotic flow (EOF) was largely reduced and the modified column can be applied to the basic proteins possessing serious adsorption property. In the 1 mM phosphate buffer (pH 5.0), the efficiency of the column for lysozyme, cytochrome C, ribonuclease A and alpha-chymotrypsinogen A in their mixture were relative high with 2.8, 4.6, 2.8 and 3.5 x 10(5) theoretical plates per meter. and RSD (n=4) expressed as migration time was relative low i.e., 1.2, 0.71, 0.60 and 1.1 (%), respectively. The column may be used in a wide pH range (pH 3-7). The above four basic proteins in the mixture have been successfully separated and the column possesses good stability. |
| 语种 | 英语 |
| WOS记录号 | WOS:000173890600014 |
| 出版者 | MARCEL DEKKER INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/79487]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Fang, M |
| 作者单位 | Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, M,Zhao, R,Shen, L,et al. Separation of four basic proteins in the mixture by capillary electrophoresis with a new chemical modification column[J]. ANALYTICAL LETTERS,2002,35(2):397-411. |
| APA | Fang, M,Zhao, R,Shen, L,Su, TS,&Liu, GQ.(2002).Separation of four basic proteins in the mixture by capillary electrophoresis with a new chemical modification column.ANALYTICAL LETTERS,35(2),397-411. |
| MLA | Fang, M,et al."Separation of four basic proteins in the mixture by capillary electrophoresis with a new chemical modification column".ANALYTICAL LETTERS 35.2(2002):397-411. |
入库方式： OAI收割
来源：化学研究所
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