Study of protein binding of acidic drug, nateglinide, in human plasma albumin solution by high performance capillary electrophoresis/frontal analysis
文献类型：期刊论文
| 作者 | Zhao, YY; Yang, GL; Fan, ZL; Wang, DX; Chen, Y |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2003-07-01 |
| 卷号 | 31期号:7页码:860-864 |
| 关键词 | Acidic Drug Nateglinide Protein Binding Parameter Capillary Electrophoresis/frontal Analysis |
| ISSN号 | 0253-3820 |
| 英文摘要 | The protein binding constants, binding sites and binding rate of acidic drug, Nateglinide, in human Plasma albumin solution was studied by high performance capillary electrophoresis frontal analysis (HPCE/FA). The experiments were carried out by determining unbound drug concentration in drug-protein equilibrium solution in a uncoated fused silica capillary (40 cm x 50 mum i.d.; effective length 32 cm) for capillary electrophoresis under the following conditions: background solution, sodium phosphate buffer solution (pH 7.4, ionic strength 0.17); ultraviolet detector, wavelength 214 nm; hydrostatic injection mode and the injection time 100 s; run voltage 18 kV. The unbound drug concentration. was calculated from the plateau height of the profile with good linear relation, r > 0.9999 for nateglinide ( n = 6). The influence of different protein concentrations on the protein binding was investigated while the drug concentration (200 mumol/L, 250 mumol/L) was fixed. Similarly, the effect of different drug concentrations on protein binding was also studied while the protein concentration ( 100 mumol/L) was fixed. The experimental data were fitted by a non-linear least-squares curve fitting program and binding parameters were obtained simultaneously. The precision of the data determined by HPCE/FA was RSD (RSD < 2.5% n = 3). The capillary electrophoresis frontal analysis presents an easy, accurate. method for pharmacokinetics and pharmacodynamics study of the drug. |
| 语种 | 英语 |
| WOS记录号 | WOS:000184609900023 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/79669]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Zhao, YY |
| 作者单位 | 1.Hebei Med Coll Continuing Educ, Expt Ctr, Baoding 071000, Peoples R China 2.Hebei Univ, Coll Chem & Environm Sci, Baoding 071002, Peoples R China 3.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, YY,Yang, GL,Fan, ZL,et al. Study of protein binding of acidic drug, nateglinide, in human plasma albumin solution by high performance capillary electrophoresis/frontal analysis[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2003,31(7):860-864. |
| APA | Zhao, YY,Yang, GL,Fan, ZL,Wang, DX,&Chen, Y.(2003).Study of protein binding of acidic drug, nateglinide, in human plasma albumin solution by high performance capillary electrophoresis/frontal analysis.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,31(7),860-864. |
| MLA | Zhao, YY,et al."Study of protein binding of acidic drug, nateglinide, in human plasma albumin solution by high performance capillary electrophoresis/frontal analysis".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 31.7(2003):860-864. |
入库方式： OAI收割
来源：化学研究所
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