Plasma-treated, collagen-anchored polylactone: Its cell affinity evaluation under shear or shear-free conditions
文献类型：期刊论文
| 作者 | Yang, J; Wan, YQ; Yang, JL; Bei, JZ; Wang, SG |
| 刊名 | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
 |
| 出版日期 | 2003-12-15 |
| 卷号 | 67A期号:4页码:1139-1147 |
| 关键词 | Polylactone Surface Modification Flow Chamber Cell Affinity Tissue Engineering |
| ISSN号 | 0021-9304 |
| DOI | 10.1002/jbm.a.10034 |
| 英文摘要 | Poly(L-lactic acid)(PLLA) and poly(L-lactic-co-glycolic acid) (PLGA) (85/15) were modified by plasma treatment. Then they were collagen anchored (PT/CA), and the cell affinity was evaluated by cell culture under shear or shear-free conditions. A convenient and "intuitionistic" dyeing method has been proposed for measuring the modified depth when plasma treatment is applied for the treatment of porous scaffolds. A parallel plate flow chamber was developed in order to study the cell affinity of a material under shear stress. Our results show that a porous scaffold can be modified by plasma treatment and that a depth of about 4.0 mm for this modification can be reached with NH3 plasma treatment (50 w, 20 Pa, 5 min). PT/CA modification is an effective surface modification method for facilitating cell transplantation and improving the cell affinity of three-dimensional porous cell scaffolds in tissue engineering. It can solve the problem of non-uniform cell distribution in most synthetic porous cell scaffolds. Using the flow chamber system, a series of quantitative data, including cell adherent fraction, cell area, and mean shape, were compared to evaluate the cell affinity of PLLA before and after PT/CA modification. The results indicate that the quality of cell attachment on PT/CA-modified PLLA apparently is better than that on unmodified PLLA. The flow chamber system potentially may be a tool for evaluating surface modification methods. (C) 2003 Wiley Periodicals, Inc. |
| 语种 | 英语 |
| WOS记录号 | WOS:000186877700009 |
| 出版者 | WILEY-LISS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/79749]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Wang, SG |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, J,Wan, YQ,Yang, JL,et al. Plasma-treated, collagen-anchored polylactone: Its cell affinity evaluation under shear or shear-free conditions[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2003,67A(4):1139-1147. |
| APA | Yang, J,Wan, YQ,Yang, JL,Bei, JZ,&Wang, SG.(2003).Plasma-treated, collagen-anchored polylactone: Its cell affinity evaluation under shear or shear-free conditions.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,67A(4),1139-1147. |
| MLA | Yang, J,et al."Plasma-treated, collagen-anchored polylactone: Its cell affinity evaluation under shear or shear-free conditions".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 67A.4(2003):1139-1147. |
入库方式： OAI收割
来源：化学研究所
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