Quartz crystal biosensor for real-time monitoring of molecular recognition between protein and small molecular medicinal agents
文献类型：期刊论文
| 作者 | Liu, Y; Yu, X; Zhao, R; Shangguan, DH; Bo, ZY; Liu, GQ |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2003-10-30 |
| 卷号 | 19期号:1页码:9-19 |
| 关键词 | Quartz Crystal Biosensor Kinetic Analysis Molecular Recognition Immunoglobulin g Sulfamethazine Sulfamethoxazole Genetic Algorithm |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/S0956-5663(03)00127-1 |
| 英文摘要 | A quartz crystal microbalance (QCM) biosensor integrated into a flow injection analysis (FIA) system was used for the real-time investigation of molecular recognition between a protein and small molecular medicinal agents. Two sulfa-drugs, sulfamethazine (SMZ) and sulfamethoxazole (SMO), were, respectively, immobilized on the gold electrodes of the piezoelectric crystals using appropriate procedures based on self-assembly of the dithiothreitol (DTT). The binding interactions of the two immobilized drug ligands, with various proteins in solution, were followed as changes in the resonant frequency of the modified crystals. Results obtained from this rapid screen analysis clearly indicated that the two drug ligands appeared quite different in this molecular recognition procedure although their structures were similar. SMZ-immobilized sensor showed specific interaction only with IgG, while SMO-imobilized sensor showed negligible specific binding with IgG, but binding with trypsin and chymotrypsin. Further studies oil the specific interaction between immobilized SMZ and three different species of IgG-human IgG, goat IgG and mouse IgG were carried Out and the marked species-dependent difference was observed. The resultant sensorgrams were rapidly analyzed by using an in-house kinetic analysis software based on genetic algorithm (GA) to derive both the kinetic rate constants (k(ass) and k(diss)) and equilibrium association constants (K-A) for IgG-SMZ interactions. For the interactions, K-A were 5.48 x 10(5), 2.75 X 10(5) and 1.86 X 10(5) M-1 for human IgG, goat IgG and mouse IgG, respectively. The kinetic data provided further insight into the structural/ functional relationships of different IgG on a molecular level. (C) 2003 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000186242000002 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/80219]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, GQ |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Y,Yu, X,Zhao, R,et al. Quartz crystal biosensor for real-time monitoring of molecular recognition between protein and small molecular medicinal agents[J]. BIOSENSORS & BIOELECTRONICS,2003,19(1):9-19. |
| APA | Liu, Y,Yu, X,Zhao, R,Shangguan, DH,Bo, ZY,&Liu, GQ.(2003).Quartz crystal biosensor for real-time monitoring of molecular recognition between protein and small molecular medicinal agents.BIOSENSORS & BIOELECTRONICS,19(1),9-19. |
| MLA | Liu, Y,et al."Quartz crystal biosensor for real-time monitoring of molecular recognition between protein and small molecular medicinal agents".BIOSENSORS & BIOELECTRONICS 19.1(2003):9-19. |
入库方式： OAI收割
来源：化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。