Determination of stachyose in Stachys Sieboldii Miq. by high performance liquid chromatography with evaporative light scattering detection
文献类型：期刊论文
| 作者 | Yin, JF; Yang, GL; Li, ZW; Liu, HY; Fan, ZL; Chen, Y |
| 刊名 | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2003-12-01 |
| 卷号 | 31期号:12页码:1409-1412 |
| 关键词 | High Performance Liquid Chromatography Evaporative Light Scattering Detection Stachyose Stachys Sieboldii Miq |
| ISSN号 | 0253-3820 |
| 英文摘要 | A method for the determination of stachyose in Stachys sieboldii miq. by high performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) was established. Two different extraction crafts were designed and compared. Stachyose was successfully separated on a Bondapak NH2 column (300 mm x 3.9 mm i.d.) at ambient temperature, the mobile phase composition was a binary solvent mixture of methanol-water (80:20, V/V). The flow rate was 1.0 mL/min. The parameters of ELSD were set as follows: drift tube temperature was 40degreesC, nitrogen carrier gas flow was 4.0 L/min. This method fulfilled all the standard requirements of linearity, accuracy and precision. The linear range was 1.02similar to12.22 mug (r=0.9995). The detection limit was 0.10 mug and the average recovery was 91.6% (RSD=1.43%). The contents of stachyose by two different extraction crafts were 29.8% and 25.2%, the purity were 63.76% and 88.35%, respectively. This method was accurate, rapid, simple and reproducible, and suitable for the integrated analysis of stachyose. |
| 语种 | 英语 |
| WOS记录号 | WOS:000188504100001 |
| 出版者 | SCIENCE CHINA PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/80683]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, GL |
| 作者单位 | 1.Coll Chem & Environm Sci, Baoding 071002, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, JF,Yang, GL,Li, ZW,et al. Determination of stachyose in Stachys Sieboldii Miq. by high performance liquid chromatography with evaporative light scattering detection[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2003,31(12):1409-1412. |
| APA | Yin, JF,Yang, GL,Li, ZW,Liu, HY,Fan, ZL,&Chen, Y.(2003).Determination of stachyose in Stachys Sieboldii Miq. by high performance liquid chromatography with evaporative light scattering detection.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,31(12),1409-1412. |
| MLA | Yin, JF,et al."Determination of stachyose in Stachys Sieboldii Miq. by high performance liquid chromatography with evaporative light scattering detection".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 31.12(2003):1409-1412. |
入库方式： OAI收割
来源：化学研究所
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