Single-mismatch detection using nucleic acid molecular 'light switch'
文献类型：期刊论文
| 作者 | Ling, LS; He, ZK; Chen, F; Zeng, YE |
| 刊名 | TALANTA
 |
| 出版日期 | 2003-02-06 |
| 卷号 | 59期号:2页码:269-275 |
| 关键词 | Molecular 'light Switch' Fluorimetry Dna Ru(Phen)(2)(Dppx)(2+) |
| ISSN号 | 0039-9140 |
| 英文摘要 | A novel nucleic acid molecular 'light switch' method is developed for the sensitive recognition and detection of a single-base mismatched oligonucleotides. The detection limit of oligonucleotide of perfect double stranded and that with single-base, two-base and three-base mismatched are 0.11, 0.17, 0.34 and 1.5 ng ml(-1), respectively. It was found that Ru(phen)(2)(d PPX)(2+) (phen = 1,10-phenanthroline, dppx = 7,8-dimethyl-dipyridophenazine) can be used to detect and recognize the perfect double stranded oligonucleotides from mismatched and random targets by the intensity of fluorescence and temperature. This method can be used to recognize and quantitatively detect target DNA with specific sequence. The advantage of this method is that no requisites are needed to separate the coexisting random targets in the case of a mixed solution containing perfect, mismatched and random targets, which make the recognition analysis of oligonucleotide simple and fast. Moreover, it has potential in the study of dynamic process of DNA hybridization. (C) 2002 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000180614100007 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/80895]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | He, ZK |
| 作者单位 | 1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ling, LS,He, ZK,Chen, F,et al. Single-mismatch detection using nucleic acid molecular 'light switch'[J]. TALANTA,2003,59(2):269-275. |
| APA | Ling, LS,He, ZK,Chen, F,&Zeng, YE.(2003).Single-mismatch detection using nucleic acid molecular 'light switch'.TALANTA,59(2),269-275. |
| MLA | Ling, LS,et al."Single-mismatch detection using nucleic acid molecular 'light switch'".TALANTA 59.2(2003):269-275. |
入库方式： OAI收割
来源：化学研究所
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